Konry Tania, Novoa Andres, Cosnier Serge, Marks Robert S
The Institute for Applied Biosciences, Ben Gurion University of the Negev, P.O. Box 653, Beer Sheva, 84105, Israel.
Anal Chem. 2003 Jun 1;75(11):2633-9. doi: 10.1021/ac026444q.
We demonstrate that it is possible to create surface-conductive fiber optics, upon which may be electropolymerized a biotinylated polypyrrole thin film, which may then be used to affinity coat the fiber with molecular recognition probes. This fiber-optic electroconductive surface modification is done by the deposition of a thin layer of indium tin oxide. Thereafter, biotin-pyrrole monomers are electropolymerized onto the conductive metal oxide surface and then exposed to avidin. Avidin-biotin interactions were used to modify the fiber optics with biotin-conjugated cholera toxin B subunit molecules, for the construction of an immunosensor to detect cholera antitoxin antibodies. The biosensor was tested for sensitivity, nonspecificity, and overall practicality.
我们证明了可以制造出表面导电的光纤，在其上面可以电聚合一层生物素化的聚吡咯薄膜，然后该薄膜可用于用分子识别探针亲和包被光纤。这种光纤导电表面改性是通过沉积一层氧化铟锡薄膜来完成的。此后，生物素 - 吡咯单体在导电金属氧化物表面上电聚合，然后与抗生物素蛋白接触。利用抗生物素蛋白 - 生物素相互作用，用生物素偶联的霍乱毒素B亚基分子修饰光纤，用于构建检测霍乱抗毒素抗体的免疫传感器。对该生物传感器进行了灵敏度、非特异性和整体实用性测试。
