Ionescu Rodica E, Gondran Chantal, Gheber Levi A, Cosnier Serge, Marks Robert S
The Institute for Applied Biosciences and Department of Biotechnology Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva, 84105, Israel.
Anal Chem. 2004 Nov 15;76(22):6808-13. doi: 10.1021/ac049413z.
The construction of amperometric immunosensors to cholera antitoxin immunoglobulins were shown to have improved sensitivity when the cholera toxin B subunit biorecognition entity was linked to an electrogenerated biotinylated polypyrrole film copolymerized with pyrrole-lactobionamide monomer. The copolymer exhibits greater film permeability than biotinylated polypyrrolic or polyphenolic films for the permeation of electroactive species. Hence, when the presence of the HRP marker of the immunoassay was determined using hydroquinone, the production of electroactive quinone was shown to permeate faster to the electrode, thus providing a faster response time.
当霍乱毒素B亚基生物识别实体与与吡咯-乳糖酰胺单体共聚的电生成生物素化聚吡咯膜相连时，构建的用于霍乱抗毒素免疫球蛋白的电流型免疫传感器显示出更高的灵敏度。对于电活性物质的渗透，该共聚物比生物素化聚吡咯或多酚膜具有更高的膜渗透性。因此，当使用对苯二酚测定免疫测定中HRP标记物的存在时，电活性醌的产生显示出更快地渗透到电极，从而提供更快的响应时间。
