准分子激光原位角膜磨镶术后泪液蛋白质模式的变化。
Changes in tear protein pattern after photorefractive keratectomy.
作者信息
Füst A, Veres A, Kiszel P, Nagy Z Z, Cervenak L, Csákány B, Maka E, Süveges I, Grus F H
机构信息
First Department of Ophthalmology, Faculty of Medicine, Semmelweis University, Budapest, Hungary.
出版信息
Eur J Ophthalmol. 2003 Jul;13(6):525-31. doi: 10.1177/112067210301300603.
PURPOSE
Changes in tear protein composition of patients who underwent photorefractive keratectomy (PRK) were analyzed.
METHODS
Tear samples were obtained from 23 eyes of 23 patients immediately before PRK and on the fourth postoperative day with glass capillaries. Tear proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Digital image analysis and evaluation of the densitometric data of the electrophoretic separations were done with BioDoc-Analyze.
RESULTS
Analysis of discriminance found a significant difference in the protein patterns (p < 0.001). This type of analysis of the electrophoretic densitographs uses all peak information simultaneously. A significant decrease (p < 0.005) in three of the main protein peaks--lactoferrin, immunoglobulin A heavy chain, and lysozyme--was also found after PRK.
CONCLUSIONS
Excimer laser ablation of the cornea has an acute effect on lacrimal gland protein secretion. Changes in tear composition may lead to feelings of dryness and to a decrease in tear film stability postoperatively.
目的
分析接受准分子激光原位角膜磨镶术（PRK）患者泪液蛋白质成分的变化。
方法
使用玻璃毛细管在PRK术前即刻及术后第4天从23例患者的23只眼中获取泪液样本。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离泪液蛋白质。使用BioDoc-Analyze对电泳分离的光密度数据进行数字图像分析和评估。
结果
判别分析发现蛋白质图谱存在显著差异（p < 0.001）。这种对电泳光密度图的分析同时使用了所有峰信息。PRK术后还发现三个主要蛋白质峰——乳铁蛋白、免疫球蛋白A重链和溶菌酶——显著降低（p < 0.005）。
结论
准分子激光角膜消融术对泪腺蛋白质分泌有急性影响。泪液成分的变化可能导致术后出现干眼感觉并降低泪膜稳定性。
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