Aivaliotis Michalis, Samolis Panagiotis, Neofotistou Elefteria, Remigy Herve, Rizos Apostolos K, Tsiotis Georgios
Division of Biochemistry, Department of Chemistry, University of Crete, Heralilion, Greece.
Biochim Biophys Acta. 2003 Sep 2;1615(1-2):69-76. doi: 10.1016/s0005-2736(03)00208-6.
The molecular size of an outer surface protein from the photosynthetic bacterium Chlorobium tepidum was studied by dynamic light scattering (DLS) and HPLC gel filtration. For that purpose, the membrane protein was isolated and studied in four different nonionic surfactants, namely t-octylphenoxypolyethenoxyethanol (Triton X-100), (methyl-6-O-(N)-heptyl-carbamoyl)-alpha-D-glucopyranoside (Hecameg), dodecyl-beta-D-maltoside (DDM) and n-octyl-oligo-oxyethylene (Octyl-POE). The protein was isolated by solubilization of the membranes with Triton X-100. The final purification step was a gel filtration, which was also used for surfactant exchange. Light scattering reveals the simultaneous presence of particles of different sizes in the 3-6 and 20-110 nm range, respectively. The smaller size is related to the hydrodynamic radius of the individual protein/surfactant complexes, whereas the larger size is associated with the presence of complex aggregates.
通过动态光散射（DLS）和高效液相色谱凝胶过滤法研究了嗜温绿菌光合细菌外表面蛋白的分子大小。为此，分离了膜蛋白，并在四种不同的非离子表面活性剂中进行研究，即叔辛基苯氧基聚乙氧基乙醇（Triton X-100）、（甲基-6-O-（N）-庚基-氨基甲酰基）-α-D-吡喃葡萄糖苷（Hecameg）、十二烷基-β-D-麦芽糖苷（DDM）和正辛基-低聚氧乙烯（Octyl-POE）。通过用Triton X-100溶解膜来分离蛋白。最终的纯化步骤是凝胶过滤，其也用于表面活性剂交换。光散射显示分别在3-6和20-110nm范围内同时存在不同大小的颗粒。较小的尺寸与单个蛋白质/表面活性剂复合物的流体动力学半径有关，而较大的尺寸与复合聚集体的存在有关。
