Frankel Adam, Li Shuwei, Starck Shelley R, Roberts Richard W
Division of Chemistry & Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Curr Opin Struct Biol. 2003 Aug;13(4):506-12. doi: 10.1016/s0959-440x(03)00110-6.
Combinatorial peptide and protein libraries have now been developed to accommodate unnatural amino acids in a genetically encoded format via in vitro nonsense and sense suppression. General translation features and specific regioselective and stereoselective properties of the ribosome endow these libraries with a broad chemical diversity. Alternatively, amino acid residues can be chemically derivatized post-translationally to add preferred functionality to the encoded peptide. All of these efforts are advancing combinatorial peptide and protein libraries for enhanced ligands against biological targets of interest.
现在已经开发出组合肽和蛋白质文库，通过体外无义抑制和有义抑制以遗传编码的形式容纳非天然氨基酸。核糖体的一般翻译特性以及特定的区域选择性和立体选择性赋予这些文库广泛的化学多样性。另外，氨基酸残基可以在翻译后进行化学衍生化，以向编码的肽添加所需的功能。所有这些努力都在推动组合肽和蛋白质文库的发展，以增强针对感兴趣的生物靶标的配体。
