Chen Tianbao, Bjourson Anthony J, McClean Stephen, Orr David F, O'Kane Edmund J, Rao Pingfan, Shaw Chris
Pharmaceutical Biotechnology Research Group, School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, Northern Ireland, UK.
Peptides. 2003 Jun;24(6):853-61. doi: 10.1016/s0196-9781(03)00167-0.
Using a novel technique that we have developed for cloning of amphibian skin secretion peptide cDNAs from lyophilized samples, we report here that maximakinin (DLPKINRKGP-bradykinin) is encoded by two different cDNAs, named BMK-1 and BMK-2, containing either four tandem repeat sequences or a single copy. The open reading frames of both precursor cDNAs were found to be 152 and 116 amino acid residues, respectively. These data provide evidence that the structural diversity of peptides in amphibian skin secretions arising from molecular evolutionary events, can be mediated by parallel diversity in encoding mRNAs that in itself may reflect serial gene duplications.
我们利用新开发的从冻干样本中克隆两栖动物皮肤分泌肽cDNA的技术，在此报告，大激肽（DLPKINRKGP - 缓激肽）由两个不同的cDNA编码，分别命名为BMK - 1和BMK - 2，它们包含四个串联重复序列或一个单拷贝。发现这两种前体cDNA的开放阅读框分别为152和116个氨基酸残基。这些数据提供了证据，表明两栖动物皮肤分泌物中肽的结构多样性源于分子进化事件，可能由编码mRNA的平行多样性介导，而这种多样性本身可能反映了基因的串联重复。
