由小肠结肠炎耶尔森菌高致病性岛编码的Irp9能够将分支酸转化为水杨酸，即耶尔森菌素的前体。
Irp9, encoded by the high-pathogenicity island of Yersinia enterocolitica, is able to convert chorismate into salicylate, the precursor of the siderophore yersiniabactin.
作者信息
Pelludat Cosima, Brem Daniela, Heesemann Jürgen
机构信息
Institut für Mikrobiologie, D-BIOL, ETHZ, 8092 Zürich, Switzerland.
出版信息
J Bacteriol. 2003 Sep;185(18):5648-53. doi: 10.1128/JB.185.18.5648-5653.2003.
Abstract
The Irp9 protein of Yersinia enterocolitica participates in the synthesis of salicylate, the precursor of the siderophore yersiniabactin. In Pseudomonas species, salicylate synthesis is mediated by two enzymes: isochorismate synthase and isochorismate pyruvate-lyase. Both enzymes are required for complementation of a Yersinia irp9 mutant. However, irp9 is not able to complement Escherichia coli entC for the production of enterobactin, which requires isochorismate as a precursor. These results suggest that Irp9 directly converts chorismate into salicylate.
摘要
小肠结肠炎耶尔森菌的Irp9蛋白参与了铁载体耶尔森菌素的前体水杨酸盐的合成。在假单胞菌属中，水杨酸盐的合成由两种酶介导：异分支酸合酶和异分支酸丙酮酸裂解酶。这两种酶都是小肠结肠炎耶尔森菌irp9突变体互补所必需的。然而，Irp9不能互补大肠杆菌entC以产生以异分支酸作为前体的肠杆菌素。这些结果表明，Irp9直接将分支酸转化为水杨酸盐。
相似文献
1
Irp9, encoded by the high-pathogenicity island of Yersinia enterocolitica, is able to convert chorismate into salicylate, the precursor of the siderophore yersiniabactin.由小肠结肠炎耶尔森菌高致病性岛编码的Irp9能够将分支酸转化为水杨酸，即耶尔森菌素的前体。
J Bacteriol. 2003 Sep;185(18):5648-53. doi: 10.1128/JB.185.18.5648-5653.2003.
2
Salicylate biosynthesis: overexpression, purification, and characterization of Irp9, a bifunctional salicylate synthase from Yersinia enterocolitica.水杨酸生物合成：小肠结肠炎耶尔森菌双功能水杨酸合酶Irp9的过表达、纯化及特性分析
J Bacteriol. 2005 Aug;187(15):5061-6. doi: 10.1128/JB.187.15.5061-5066.2005.
3
Lysine221 is the general base residue of the isochorismate synthase from Pseudomonas aeruginosa (PchA) in a reaction that is diffusion limited.赖氨酸 221 是铜绿假单胞菌（PchA）异分支酸合酶的通用碱基残基，该反应受扩散限制。
Arch Biochem Biophys. 2013 Oct 1;538(1):49-56. doi: 10.1016/j.abb.2013.07.026. Epub 2013 Aug 11.
4
Crystal structure of Escherichia coli enterobactin-specific isochorismate synthase (EntC) bound to its reaction product isochorismate: implications for the enzyme mechanism and differential activity of chorismate-utilizing enzymes.大肠杆菌肠杆菌素特异性异分支酸合酶（EntC）与反应产物异分支酸结合的晶体结构：对酶机制和分支酸利用酶的差异活性的影响。
J Mol Biol. 2010 Mar 19;397(1):290-300. doi: 10.1016/j.jmb.2010.01.019. Epub 2010 Jan 15.
5
Crystal structures of Yersinia enterocolitica salicylate synthase and its complex with the reaction products salicylate and pyruvate.小肠结肠炎耶尔森菌水杨酸合酶及其与反应产物水杨酸和丙酮酸复合物的晶体结构。
J Mol Biol. 2006 Mar 24;357(2):524-34. doi: 10.1016/j.jmb.2005.12.078. Epub 2006 Jan 11.
6
Mechanistic and inhibition studies of chorismate-utilizing enzymes.分支酸利用酶的机制与抑制研究。
Biochem Soc Trans. 2005 Aug;33(Pt 4):763-6. doi: 10.1042/BST0330763.
7
The structure of MbtI from Mycobacterium tuberculosis, the first enzyme in the biosynthesis of the siderophore mycobactin, reveals it to be a salicylate synthase.来自结核分枝杆菌的MbtI（铁载体分枝杆菌素生物合成中的第一种酶）的结构表明它是一种水杨酸合酶。
J Bacteriol. 2006 Sep;188(17):6081-91. doi: 10.1128/JB.00338-06.
8
Redesign of MST enzymes to target lyase activity instead promotes mutase and dehydratase activities.重新设计 MST 酶以靶向裂解酶活性反而促进了变位酶和脱水酶的活性。
Arch Biochem Biophys. 2013 Nov 1;539(1):70-80. doi: 10.1016/j.abb.2013.09.007. Epub 2013 Sep 19.
9
Functional analysis of yersiniabactin transport genes of Yersinia enterocolitica.小肠结肠炎耶尔森菌耶尔辛菌素转运基因的功能分析
Microbiology (Reading). 2001 May;147(Pt 5):1115-1127. doi: 10.1099/00221287-147-5-1115.
10
Structural genes for salicylate biosynthesis from chorismate in Pseudomonas aeruginosa.铜绿假单胞菌中由分支酸合成水杨酸的结构基因。
Mol Gen Genet. 1995 Nov 15;249(2):217-28. doi: 10.1007/BF00290369.
引用本文的文献
1
The genetic orchestra of salicylic acid in plant resilience to climate change induced abiotic stress: critical review.水杨酸在植物应对气候变化诱导的非生物胁迫中的遗传调控：综述
Stress Biol. 2024 Jun 17;4(1):31. doi: 10.1007/s44154-024-00160-2.
2
Diversity and functional traits of indigenous soil microbial flora associated with salinity and heavy metal concentrations in agricultural fields within the Indus Basin region, Pakistan.巴基斯坦印度河流域地区农田中与盐分和重金属浓度相关的本土土壤微生物群落的多样性和功能特征。
Front Microbiol. 2022 Nov 7;13:1020175. doi: 10.3389/fmicb.2022.1020175. eCollection 2022.
3
Microenvironmental Factors that Shape Bacterial Metabolites in Inflammatory Bowel Disease.影响炎症性肠病中细菌代谢物的微环境因素。
Front Cell Infect Microbiol. 2022 Jul 15;12:934619. doi: 10.3389/fcimb.2022.934619. eCollection 2022.
4
Complete, closed and curated genome sequences of subsp. isolates from Australia indicate mobilome-driven localized evolution and novel pathogenicity determinants.来自澳大利亚的 亚种分离株的完整、封闭和精选基因组序列表明，可移动基因组驱动了局部进化和新的致病性决定因素。
Microb Genom. 2021 Apr;7(4). doi: 10.1099/mgen.0.000562.
5
De novo design of an intercellular signaling toolbox for multi-channel cell-cell communication and biological computation.用于多通道细胞间通信和生物计算的细胞间信号工具箱的从头设计。
Nat Commun. 2020 Aug 24;11(1):4226. doi: 10.1038/s41467-020-17993-w.
6
Engineering Heterologous Production of Salicylate Glucoside and Glycosylated Variants.水杨酸葡萄糖苷及糖基化变体的工程异源生产
Front Microbiol. 2018 Sep 20;9:2241. doi: 10.3389/fmicb.2018.02241. eCollection 2018.
7
Uropathogenic enterobacteria use the yersiniabactin metallophore system to acquire nickel.尿路致病性肠杆菌利用耶尔森菌生物素金属载体系统获取镍。
J Biol Chem. 2018 Sep 28;293(39):14953-14961. doi: 10.1074/jbc.RA118.004483. Epub 2018 Aug 14.
8
An E. coli biosensor for screening of cDNA libraries for isochorismate pyruvate lyase-encoding cDNAs.用于筛选异柠檬酸丙酮酸裂解酶编码 cDNA 文库的大肠杆菌生物传感器。
Mol Genet Genomics. 2018 Oct;293(5):1181-1190. doi: 10.1007/s00438-018-1450-5. Epub 2018 May 23.
9
Corynebacterium glutamicum as platform for the production of hydroxybenzoic acids.谷氨酸棒杆菌作为生产羟基苯甲酸的平台。
Microb Cell Fact. 2018 May 12;17(1):70. doi: 10.1186/s12934-018-0923-x.
10
Comparative analysis of plant isochorismate synthases reveals structural mechanisms underlying their distinct biochemical properties.比较植物异分支酸合成酶的分析揭示了其独特生化特性的结构机制。
Biosci Rep. 2018 Mar 9;38(2). doi: 10.1042/BSR20171457. Print 2018 Apr 27.
本文引用的文献
1
Salicylate biosynthesis in Pseudomonas aeruginosa. Purification and characterization of PchB, a novel bifunctional enzyme displaying isochorismate pyruvate-lyase and chorismate mutase activities.铜绿假单胞菌中的水杨酸生物合成。新型双功能酶PchB的纯化与特性鉴定，该酶具有异分支酸丙酮酸裂解酶和分支酸变位酶活性。
J Biol Chem. 2002 Jun 14;277(24):21768-75. doi: 10.1074/jbc.M202410200. Epub 2002 Apr 5.
2
Clustering of isochorismate synthase genes menF and entC and channeling of isochorismate in Escherichia coli.大肠杆菌中异分支酸合酶基因menF和entC的聚类以及异分支酸的通道化作用
Biochim Biophys Acta. 2001 Dec 30;1522(3):151-7. doi: 10.1016/s0167-4781(01)00325-6.
3
Functional analysis of yersiniabactin transport genes of Yersinia enterocolitica.小肠结肠炎耶尔森菌耶尔辛菌素转运基因的功能分析
Microbiology (Reading). 2001 May;147(Pt 5):1115-1127. doi: 10.1099/00221287-147-5-1115.
4
Analysis of the pmsCEAB gene cluster involved in biosynthesis of salicylic acid and the siderophore pseudomonine in the biocontrol strain Pseudomonas fluorescens WCS374.对生防菌株荧光假单胞菌WCS374中参与水杨酸和铁载体假单胞菌素生物合成的pmsCEAB基因簇的分析。
J Bacteriol. 2001 Mar;183(6):1909-20. doi: 10.1128/JB.183.6.1909-1920.2001.
5
Common and specific characteristics of the high-pathogenicity island of Yersinia enterocolitica.小肠结肠炎耶尔森菌高致病性岛的共同特征与特异性特征
Infect Immun. 1999 Oct;67(10):5265-74. doi: 10.1128/IAI.67.10.5265-5274.1999.
6
Iron acquisition and metabolism by mycobacteria.分枝杆菌的铁摄取与代谢
J Bacteriol. 1999 Aug;181(15):4443-51. doi: 10.1128/JB.181.15.4443-4451.1999.
7
The high-pathogenicity island of Yersinia pseudotuberculosis can be inserted into any of the three chromosomal asn tRNA genes.假结核耶尔森菌的高致病性岛可插入三个染色体天冬酰胺tRNA基因中的任何一个。
Mol Microbiol. 1998 Dec;30(5):965-78. doi: 10.1046/j.1365-2958.1998.01124.x.
8
Identification of a Mycobacterium tuberculosis gene cluster encoding the biosynthetic enzymes for assembly of the virulence-conferring siderophore mycobactin.鉴定一个编码用于组装赋予毒力的铁载体分枝杆菌素的生物合成酶的结核分枝杆菌基因簇。
Chem Biol. 1998 Nov;5(11):631-45. doi: 10.1016/s1074-5521(98)90291-5.
9
Iron acquisition in plague: modular logic in enzymatic biogenesis of yersiniabactin by Yersinia pestis.鼠疫中的铁获取：鼠疫耶尔森菌耶尔森菌素酶促生物合成中的模块化逻辑
Chem Biol. 1998 Oct;5(10):573-86. doi: 10.1016/s1074-5521(98)90115-6.
10
The yersiniabactin biosynthetic gene cluster of Yersinia enterocolitica: organization and siderophore-dependent regulation.小肠结肠炎耶尔森菌的耶尔森菌素生物合成基因簇：组织与铁载体依赖性调控
J Bacteriol. 1998 Feb;180(3):538-46. doi: 10.1128/JB.180.3.538-546.1998.
Zotero 插件