Koehler Andreas, Karch Helge, Beikler Thomas, Flemmig Thomas F, Suerbaum Sebastian, Schmidt Herbert
Institut für Hygiene und Mikrobiologie der Bayerischen Julius-Maximilians-Universität, 97080 Würzburg, Germany.
Institut für Hygiene, 48149 Münster, Germany.
Microbiology (Reading). 2003 Sep;149(Pt 9):2407-2415. doi: 10.1099/mic.0.26267-0.
In this study, the genetic relationship of 19 Porphyromonas gingivalis isolates from patients with periodontitis was investigated by multilocus sequence analysis. Internal 400-600 bp DNA fragments of the 10 chromosomal genes ef-tu, ftsQ, hagB, gpdxJ, pepO, mcmA, dnaK, recA, pga and nah were amplified by PCR and sequenced. No two isolates were identical at all 10 loci. Phylogenetic analyses indicated a panmictic population structure of P. gingivalis. Split decomposition analysis, calculation of homoplasy ratios and analyses of clustered polymorphisms all indicate that recombination plays a major role in creating the genetic heterogeneity of P. gingivalis. A standardized index of association of 0.0898 indicates that the P. gingivalis genes analysed are close to linkage equilibrium.
