Baldy-Chudzik Katarzyna, Niedbach Joanna, Stosik Michał
Institute of Biotechnology and Environmental Sciences, University of Zielona Góra, Monte Cassino 21b, 65-561 Zielona Góra, Poland.
Cell Mol Biol Lett. 2003;8(3):793-8.
The rep-PCR fingerprinting method, with the support of ERIC and REP primers, was used to analyse the genomic diversity of 93 E. coli strains isolated from lake water samples drawn at two different depths. The applied UPGMA for DNA analysis did not reveale any genomic similarities between the 48 E. coli strains derived from the subsurface-zone water and the 43 of the bottom-zone water. The considerable genomic diversity of the E. coli of the surface zone was expressed as a dendrogram in the form of 8 similarity groups comprising strains isolated from samples drawn over one month. The bottom-zone strains, which display a lesser degree of genomic diversity (5 similarity groups), showed distinct common features in their DNA fingerprints. In the similarity dendrogram for the bottom-zone, strains derived in different months of sampling were segregated into the same similarity groups. Applying REP primers in rep-PCR generates more complex fingerprints increasing the discriminatory power of the analysis, whereas the ERIC primer generates less complex fingerprint patterns, and is thus clearer to interpret.
在ERIC和REP引物的支持下，采用重复聚合酶链反应指纹图谱法分析了从两个不同深度采集的湖水样本中分离出的93株大肠杆菌的基因组多样性。应用UPGMA进行DNA分析时，未发现从次表层水样中分离出的48株大肠杆菌与底层水样中分离出的43株大肠杆菌之间存在任何基因组相似性。表层区域大肠杆菌的显著基因组多样性以树形图的形式表示为8个相似性组，这些相似性组包含在一个多月的时间里从样本中分离出的菌株。底层区域的菌株基因组多样性程度较低（5个相似性组），在其DNA指纹图谱中显示出明显的共同特征。在底层区域的相似性树形图中，不同采样月份分离出的菌株被归入相同的相似性组。在重复聚合酶链反应中应用REP引物会产生更复杂的指纹图谱，从而提高分析的鉴别力，而ERIC引物产生的指纹图谱模式较简单，因此更易于解读。
