Tkaczuk J, Abbal M, Modesto-Segonds A, Llovera J J, Rostaing L, Durand D, Suc J M, Ohayon E
Laboratoire d'Immunologie, CHU Rangueil, Toulouse.
Presse Med. 1992 Dec 2;21(41):1991-3.
We report characterization of CD45 isoforms expressed by CD8+ lymphocytes in peripheral blood and in the graft of 40 kidney transplanted patients who underwent kidney biopsy on the basis of clinical signs suggesting rejection. Standard histological examination of the biopsy fragments and three-color cytofluorimetric analysis of lymphocytes extracted from the same fragments by mechanical and enzymatic treatment were performed simultaneously and compared to the peripheral blood lymphocytes. In 14/40 biopsies where lymphocyte extraction succeeded, the predominant subset was CD8 (CD4/CD8 mean ratio was 0.53). Almost all CD8+ cells were activated: among these CD8+ cells, 55 percent were HLA-DR+, and 68 percent CD45RO+, i.e. of a memory cell type with cytotoxic activity. This situation resembles the in vitro observation made during mitogenic stimulation of lymphocytes by phytohemagglutinin, OKT3 or CML ("culture mixte lymphocytaire"). Beside their evident interest for the diagnosis, these data could be useful for our understanding of the physiopathology of the rejection crisis.
我们报告了40例接受肾活检的肾移植患者外周血及移植物中CD8+淋巴细胞所表达的CD45异构体的特征。这些患者因临床体征提示排斥反应而接受肾活检。对活检组织碎片进行标准组织学检查，并对通过机械和酶处理从同一组织碎片中提取的淋巴细胞进行三色细胞荧光分析，同时与外周血淋巴细胞进行比较。在14例成功提取淋巴细胞的活检组织（共40例）中，主要亚群为CD8（CD4/CD8平均比值为0.53）。几乎所有CD8+细胞均被激活：在这些CD8+细胞中，55%为HLA-DR+，68%为CD45RO+，即具有细胞毒性活性的记忆细胞类型。这种情况类似于在淋巴细胞受到植物血凝素、OKT3或慢性髓细胞白血病（“混合淋巴细胞培养”）有丝分裂刺激期间的体外观察结果。除了对诊断具有明显意义外，这些数据可能有助于我们理解排斥反应危机的生理病理学。
