Razavi S, Nasr-Esfahani M H, Mardani M, Mafi A, Moghdam A
Department of Anatomy, Isfahan University of Medical Sciences, Isfahan, Iran.
Andrologia. 2003 Aug;35(4):238-43. doi: 10.1046/j.1439-0272.2003.00566.x.
The aim of this study was to evaluate the effect of sperm chromatin anomalies on fertilization outcome post-intracytoplasmic sperm injection (ICSI). Therefore, along with semen parameters, Chromomycin A3 (CMA3) staining for protamine deficiency, aniline blue staining for excessive histones, SDS for sperm chromatin stability and SDS + EDTA for the ability of sperm to undergo decondensation were carried out on 55 semen samples from patients referred to the Isfahan Fertility and Infertility Center for ICSI. The results showed that among the aforementioned tests and semen parameters only CMA3 showed a significant correlation with fertilization outcome post-ICSI. Patients were also grouped according to CMA3 level of <30% or >30% or fertilization rate of <50% or >50%. The results show that the mean percentage fertilization and mean percentage of CMA3 positivity is different in both groups, respectively. The area under receiver operator characteristics curve shows that CMA3 is a highly sensitive and specific test for prediction of fertilization outcome post-ICSI. In conclusion, that sperm protamine deficiency has profound effect on fertilization failure in ICSI.
本研究的目的是评估精子染色质异常对卵胞浆内单精子注射（ICSI）后受精结果的影响。因此，除了精液参数外，还对转诊至伊斯法罕生育与不孕中心接受ICSI的55份精液样本进行了用于检测鱼精蛋白缺乏的Chromomycin A3（CMA3）染色、用于检测组蛋白过量的苯胺蓝染色、用于检测精子染色质稳定性的SDS以及用于检测精子去浓缩能力的SDS + EDTA。结果显示，在上述检测和精液参数中，只有CMA3与ICSI后的受精结果存在显著相关性。患者还根据CMA3水平<30%或>30%或受精率<50%或>50%进行分组。结果表明，两组的平均受精百分比和CMA3阳性平均百分比分别不同。受试者工作特征曲线下面积表明，CMA3是预测ICSI后受精结果的高度敏感和特异的检测方法。总之，精子鱼精蛋白缺乏对ICSI中的受精失败有深远影响。
