Edwards Malcolm J, Thomas R C, Wong Y L
University of Wales Institute, School of Applied Sciences, Llandaf Campus, Cardiff, UK.
J Cutan Pathol. 2003 Sep;30(8):479-85. doi: 10.1034/j.1600-0560.2003.00004.x.
The aberrant expression of both the retinoblastoma and p53 tumor suppressor genes has been associated with more aggressive tumors, metastasis and lower survival.
We have evaluated immunohistochemically the expression of pRB in a panel of non-melanoma skin cancers containing p53 somatic mutations.
Nuclear anti-p53 staining was detected in 18 (72%) differentiated squamous cell carcinomas, six (100%) undifferentiated squamous cell carcinomas and seven (28%) basal cell carcinomas. A correlation was observed between p53 expression and the proliferative activity of differentiated squamous cell carcinomas (P < 0.066), undifferentiated squamous cell carcinomas (P < 0.05) and basal cell carcinomas (P < 0.01). Tumors were selected for mutant p53 expression by PCR-directed DNA sequencing and pRB expression measured immunohistochemically. Anti-pRB reactivity was detected in the nuclei of basal and suprabasal layer cells of normal epidermis, and in the proliferative compartment of all the differentiated squamous cell carcinomas, and basal cell carcinomas. A correlation was observed between pRB expression and the proliferative activity of the differentiated squamous cell carcinomas (P < 0.01) and basal cell carcinomas (P < 0.025). However, anti-pRB reactivity was not detected in the six anti-p53 reactive undifferentiated squamous cell carcinomas.
视网膜母细胞瘤和p53肿瘤抑制基因的异常表达与更具侵袭性的肿瘤、转移及较低的生存率相关。
我们采用免疫组织化学方法评估了一组含有p53体细胞突变的非黑素瘤皮肤癌中pRB的表达。
在18例（72%）分化型鳞状细胞癌、6例（100%）未分化型鳞状细胞癌和7例（28%）基底细胞癌中检测到核抗p53染色。在分化型鳞状细胞癌（P < 0.066）、未分化型鳞状细胞癌（P < 0.05）和基底细胞癌（P < 0.01）中观察到p53表达与增殖活性之间存在相关性。通过聚合酶链反应定向DNA测序选择具有突变型p53表达的肿瘤，并采用免疫组织化学方法检测pRB表达。在正常表皮的基底层和基底上层细胞的细胞核中，以及在所有分化型鳞状细胞癌和基底细胞癌的增殖区中检测到抗pRB反应性。在分化型鳞状细胞癌（P < 0.01）和基底细胞癌（P < 0.025）中观察到pRB表达与增殖活性之间存在相关性。然而，在6例抗p53反应性未分化型鳞状细胞癌中未检测到抗pRB反应性。
