Peclaris G M, Pappa A, Deligiannis K, Koutsotolis K
Laboratory of Physiology of Reproduction, Department of Animal Production, Faculty of Agriculture, Aristotle University, Thessaloniki, Macedonia, Greece.
Reprod Domest Anim. 2003 Oct;38(5):367-72. doi: 10.1046/j.1439-0531.2003.00429.x.
The development of competitive enzyme immunoassays for ovine plasma LH (oLH) and FSH (oFSH) is described. Standards and plasma samples were preincubated with diluted antiserum to oLH or oFSH and the reacted solution (100 micro l per well) was transferred to plates previously coated with oLH or oFSH, respectively. The second antibody used was anti-rabbit IgG horseradish peroxidase. The measuring range was 0.39-50 ng/ml for each hormone and the 50% relative binding sensitivity was 9 ng/ml for oLH. The respective value for oFSH was 3.5 or 34 ng/ml with different hormone and antibody preparations used for the assay. The enzyme immunoassays were used to determine oLH and oFSH levels in plasma from ewes of two breeds during the oestrous cycle. The assays detected the first FSH surge coincident with the LH surge, the second FSH surge about 24 h later and the periodic fluctuations of FSH concentrations during the luteal phase of the oestrous cycle. These enzyme immunoassays are an efficient and economic alternative to the established radioimmunoassays (RIA) for oLH and oFSH.
本文描述了用于检测绵羊血浆促黄体生成素（oLH）和促卵泡素（oFSH）的竞争性酶免疫分析方法的开发。标准品和血浆样本先与稀释的抗oLH或抗oFSH抗血清预孵育，然后将反应溶液（每孔100微升）分别转移至预先包被有oLH或oFSH的酶标板中。所用的第二抗体为辣根过氧化物酶标记的抗兔IgG。每种激素的检测范围为0.39 - 50纳克/毫升，oLH的50%相对结合灵敏度为9纳克/毫升。对于oFSH，使用不同的激素和抗体制剂进行检测时，其相应值为3.5或34纳克/毫升。该酶免疫分析方法用于测定两个品种母羊发情周期血浆中的oLH和oFSH水平。这些检测方法检测到首次FSH峰与LH峰同时出现，第二次FSH峰约在24小时后出现，以及发情周期黄体期FSH浓度的周期性波动。这些酶免疫分析方法是已有的oLH和oFSH放射免疫分析（RIA）的一种高效且经济的替代方法。
