Oo M M, Win K M, Naing K M, Kaneda Y, Tanaka T
Research Division of Parasitology, Department of Medical Research, Yangon, Union of Myanmar.
Trop Geogr Med. 1992 Oct;44(4):355-8.
Amoebae were isolated from an arthritic knee of a male patient. The organisms grew in Egg Yolk Infusion medium, and in Diamond's Biosate Iron-Serum-33 medium, which contained Trypanosoma cruzi, and were identified microscopically as Entamoeba histolytica. Furthermore, amoebae-like organisms in aspirate and cultures were immunocytochemically identified using monoclonal antibodies against E. histolytica. On the basis of the morphologic and immunologic observations, it was concluded that the knee lesion was caused by infection with E. hystolytica. The knee lesion resolved following treatment with metronidazole. This case is the first report of an amoebic infection of the knee joint.
从一名男性患者的关节炎膝关节中分离出变形虫。这些生物体在蛋黄输注培养基以及含有克氏锥虫的戴蒙德生物盐铁 - 血清 - 33培养基中生长，并通过显微镜鉴定为溶组织内阿米巴。此外，使用针对溶组织内阿米巴的单克隆抗体对抽吸物和培养物中的类阿米巴生物体进行了免疫细胞化学鉴定。基于形态学和免疫学观察结果，得出结论：膝关节病变是由溶组织内阿米巴感染引起的。甲硝唑治疗后膝关节病变得到缓解。该病例是膝关节阿米巴感染的首例报告。
