Chen J, Rodriguez J A, Barnett B, Hashimoto N, Tang J, Yoneda T
Department of Pediatric Dentistry, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229, USA.
Connect Tissue Res. 2003;44 Suppl 1:279-84.
The present study was conducted to determine the effects of bone sialoprotein (BSP) in promoting vascular invasion of tumor cells in metastasis. We used a Matrigel system and the MDA-231 human breast cancer cells transfected with human BSP cDNA (MDA-231/BSP). Quantative analysis indicated an average of 1.7-fold increase in cell numbers that migrated through the endothelial cells in MDA-231/BSP cells compared with empty vector-transfected MDA-231 cells (MDA-231/EV). In an in vivo assay, the MDA-231 cells were incubated with or without BSP antibodies and were then inoculated onto the upper chorioallantoic membrane (CAM) of chicken embryos, in which the only route for the tumor cells to reach the lower CAM was to migrate through the embryonic vasculature. PCR amplification using human Alu primers and genomic DNA from harvested lower CAM showed an average reduction of 67% in the samples treated with BSP antibodies. These preliminary data suggest that, in metastasis, BSP may enhance the penetrating ability of tumor cells through endothelial cells and basement membrane into blood vessels. BSP antibodies can specifically hinder this effect in an in vivo system.
本研究旨在确定骨唾液蛋白（BSP）在促进肿瘤细胞转移过程中血管侵袭方面的作用。我们使用了基质胶系统以及转染了人BSP cDNA的MDA - 231人乳腺癌细胞（MDA - 231/BSP）。定量分析表明，与空载体转染的MDA - 231细胞（MDA - 231/EV）相比，MDA - 231/BSP细胞中穿过内皮细胞迁移的细胞数量平均增加了1.7倍。在体内试验中，将MDA - 231细胞与BSP抗体一起或不与BSP抗体一起孵育，然后接种到鸡胚的上绒毛尿囊膜（CAM）上，肿瘤细胞到达下CAM的唯一途径是通过胚胎血管迁移。使用人Alu引物和从收获的下CAM提取的基因组DNA进行PCR扩增显示，用BSP抗体处理的样品平均减少了67%。这些初步数据表明，在转移过程中，BSP可能增强肿瘤细胞穿过内皮细胞和基底膜进入血管的穿透能力。BSP抗体在体内系统中可特异性地阻碍这种作用。
