低渗、交联和交联透化大鼠红细胞作为潜在载体系统的特性

Properties of hypotonized, crosslinked and crosslinked- permeabilized rat erythrocytes as potential carrier systems.

作者信息

Luque J, Garín M I, Sanz S, Ropero P, Pinilla M

机构信息

Departamento de Bioquímica y Biologia Molecular, Universidad de Alcalá, Madrid, Spain.

出版信息

Adv Exp Med Biol. 1992;326:81-9. doi: 10.1007/978-1-4615-3030-5_10.

DOI:10.1007/978-1-4615-3030-5_10

PMID:1295328

Abstract

The osmotic fragility curves of isotonic rat RBCs, studied at pH 8 to avoid the Hb insolubility, are similar to those in humans at pH 7.4. Hypotonized rat RBCs, either directly or dialysed (0-24 h), are more hemolysis-resistant than isotonic rat RBCs. The discocyte-stomatocyte-spherocyte transformation can be observed with scanning electron microscopy. Protein crosslinking with dimethyl suberimidate can stabilize RBCs. The crosslinking level (60%), the cellular yield (80%), the mechanical and hemolytic resistance and the protective effect of enzyme activities, were studied in crosslinked or crosslinked- permeabilized RBCs after digitonin treatment. The normal discocytic shape of RBCs under scanning electron microscopy becomes stomatocytic in crosslinked and crosslinked- permeabilized RBCs with an erosioned surface.

摘要

为避免血红蛋白不溶性，在pH 8条件下研究的等渗大鼠红细胞的渗透脆性曲线与pH 7.4条件下人类的相似。直接或透析（0 - 24小时）的低渗大鼠红细胞比等渗大鼠红细胞更具抗溶血能力。通过扫描电子显微镜可观察到盘状红细胞 - 口形红细胞 - 球形红细胞的转变。用辛二酸二甲酯进行蛋白质交联可使红细胞稳定。研究了在洋地黄皂苷处理后的交联或交联 - 透化红细胞中的交联水平（60%）、细胞产量（80%）、机械和溶血抗性以及酶活性的保护作用。在扫描电子显微镜下，正常的盘状红细胞形态在交联和交联 - 透化的红细胞中变成口形，且表面有侵蚀。