Cao Wen-gen, Chen Lei, Jiao Qing-cai, Ding Li-hua
Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, Nanjing 210093, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2003 Jun;23(3):587-90.
The spatial orientation interaction mechanism of chondroitin sulfate (CS) and azure A (AA) was studied by spectrometry. The maximum binding number (N = 151) and the binding equilibrium constant (K = 5. 24 x 10(4)) were obtained. The molecular binding model of the interaction between AA and CS was established. The influence of the molar ratio of AA/CS, ethanol, hydroxypropyl-beta-cyclodextrin, triton X-100 and NaCl on the spectra of AA-CS complex was investigated. We conclude that the color change of complex depends on not only the electrostatic interaction between AA and CS but also the density of AA binding on CS. And the formation of the absorption peak at 550 nm and the color change of complex result mainly from the hydrophobic interaction between AA and AA binding on CS.
采用光谱法研究了硫酸软骨素（CS）与天青A（AA）的空间取向相互作用机制。获得了最大结合数（N = 151）和结合平衡常数（K = 5.24×10⁴）。建立了AA与CS相互作用的分子结合模型。研究了AA/CS的摩尔比、乙醇、羟丙基-β-环糊精、曲拉通X-100和NaCl对AA-CS复合物光谱的影响。我们得出结论，复合物的颜色变化不仅取决于AA与CS之间的静电相互作用，还取决于AA在CS上的结合密度。并且在550nm处吸收峰的形成以及复合物的颜色变化主要源于AA与CS上结合的AA之间的疏水相互作用。
