离子型谷氨酸受体拮抗剂可抑制向大鼠中脑导水管周围灰质背外侧注射一氧化氮供体所产生的厌恶效应。
Ionotropic glutamate-receptor antagonists inhibit the aversive effects of nitric oxide donor injected into the dorsolateral periaqueductal gray of rats.
作者信息
Moreira Fabrício Araújo, Molchanov Moriana Ludmilla, Guimarães Francisco Silveira
机构信息
Department of Pharmacology, FMRP, Campus USP, 14049-900, Ribeirão Preto, SP, Brazil.
出版信息
Psychopharmacology (Berl). 2004 Jan;171(2):199-203. doi: 10.1007/s00213-003-1569-9. Epub 2003 Aug 30.
RATIONALE
Nitric oxide (NO) is a gas neurotransmitter that may facilitate glutamate release in the central nervous system. NO donors or glutamate agonists injected into the dorsolateral periaqueductal grey (dlPAG) induce flight behaviour.
OBJECTIVES
To test the hypothesis that the defensive reactions induced by an NO donor in the dlPAG would be attenuated by pretreatment with AMPA/kainate or NMDA glutamate receptor antagonists.
METHODS
Male Wistar rats with cannulae aimed at the dlPAG received vehicle, AP7 (a NMDA receptor antagonist, 2 nmol) or NBQX (an AMPA/kainite receptor antagonist, 100 nmol) injection 10 min before the administration of SIN-1 (an NO donor, 300 nmol). Immediately after the last injection, their behavior was observed in an open arena during 10 min.
RESULTS
SIN-1 induced flight reactions characterized by running and jumps. Pretreatment with AP7 or NBQX completely prevented the effects of SIN-1.
CONCLUSION
The results suggest that the aversive reactions induced by an NO donor in the dlPAG depend on ionotropic glutamate receptor activation.
理论依据
一氧化氮（NO）是一种气体神经递质，可能会促进中枢神经系统中谷氨酸的释放。向背外侧导水管周围灰质（dlPAG）注射NO供体或谷氨酸激动剂会诱发逃避行为。
目的
检验以下假设：用AMPA/海人藻酸或NMDA谷氨酸受体拮抗剂预处理可减弱dlPAG中NO供体诱导的防御反应。
方法
将插管对准dlPAG的雄性Wistar大鼠在给予SIN-1（一种NO供体，300 nmol）前10分钟接受溶剂、AP7（一种NMDA受体拮抗剂，2 nmol）或NBQX（一种AMPA/海人藻酸受体拮抗剂，100 nmol）注射。最后一次注射后立即在开放场地观察它们10分钟的行为。
结果
SIN-1诱发了以奔跑和跳跃为特征的逃避反应。用AP7或NBQX预处理可完全阻止SIN-1的作用。
结论
结果表明，dlPAG中NO供体诱导的厌恶反应取决于离子型谷氨酸受体的激活。
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