Uchida A, Yoshida T, Ogawa M, Nagasawa T
Department of Biomolecular Science, Gifu University, Yanagido 1-1, 501-1193 Gifu, Japan.
Appl Microbiol Biotechnol. 2003 Sep;62(4):337-41. doi: 10.1007/s00253-003-1248-0. Epub 2003 Feb 26.
Microorganisms aerobically degrading quinolinic acid, lutidinic acid or isocinchomeronic acid were isolated and the microbial regioselective hydroxylation of these pyridine dicarboxylic acids was studied. Alcaligenes sp. UK21 cells converted quinolinic acid into 6-hydroxypicolinic acid, suggesting the involvement of two enzyme reactions catalyzing hydroxylation at position C6 and decarboxylation at position C3 of quinolinic acid. Resting cells of Alcaligenes sp. UK21 accumulated 94.9 mM 6-hydroxypicolinic acid (13.2 g l(-1)), with a 96% molar conversion yield by 48 h incubation. Rhizobium sp. LA17 and Hydrogenophaga sp. IMA01 catalyzed the regioselective hydroxylation of lutidinic acid and isocinchomeronic acid into 6-hydroxylutidinic acid and 6-hydroxyisocinchomeronic acid, respectively. 6-Hydroxylutidinic acid accumulated up to 95.4 mM (17.5 g l(-1)) by 24 h incubation in the resting cells reaction, using Rhizobium sp. LA17, with a 99% molar conversion yield. Resting cells of Hydrogenophaga sp. IMA01 produced 88.7 mM 6-hydroxyisocinchomeronic acid (16.2 g l(-1)) by 24 h incubation, with a 81% molar conversion yield.
分离出了需氧降解喹啉酸、二甲基吡啶酸或异喹啉酸的微生物，并研究了这些吡啶二羧酸的微生物区域选择性羟基化作用。产碱杆菌属UK21细胞将喹啉酸转化为6-羟基吡啶甲酸，这表明存在两种酶反应，一种催化喹啉酸C6位的羟基化，另一种催化C3位的脱羧反应。产碱杆菌属UK21的静息细胞在48小时培养后积累了94.9 mM的6-羟基吡啶甲酸（13.2 g l(-1)），摩尔转化率为96%。根瘤菌属LA17和嗜氢菌属IMA01分别催化二甲基吡啶酸和异喹啉酸的区域选择性羟基化，生成6-羟基二甲基吡啶酸和6-羟基异喹啉酸。在根瘤菌属LA17的静息细胞反应中，通过24小时培养，6-羟基二甲基吡啶酸积累量高达95.4 mM（17.5 g l(-1)），摩尔转化率为99%。嗜氢菌属IMA01的静息细胞在24小时培养后产生了88.7 mM的6-羟基异喹啉酸（16.2 g l(-1)），摩尔转化率为81%。
