Zhang T, Song S, Li L, Qi W, Hu W, Wang P, Chen K, Fang P
Second Teaching Hospital, Tianjin Medical College.
Yi Chuan Xue Bao. 1992;19(5):410-5.
The messenger RNA was extracted from bovine anterior pituitary glands and was purified by oligo(dT)-cellulose chromatography. The length of the mRNA was 1,200 nucleotides measured by agarose gel electrophoresis containing methylmercury hydroxide. The bovine prolactin (PRL) mRNA was confirmed by Northern blot analysis of the mRNA with gamma-32P labelled synthetic oligonucleotide probes based on the partial amino acid sequences of bovine PRL and could stimulate the incorporation of 35S-methionine into protein in the rabbit reticulocyte cell-free system. The translation product of bovine PRL mRNA was immunoprecipitable by rabbit anti-ovine PRL anti-antibodies. The molecular weight of the translation product corresponding to the bovine PRL precursor was estimated to be approximately 25,000 by SDS polyacrylamide gel electrophoresis and autoradiograph.
从牛垂体前叶提取信使核糖核酸（mRNA），并通过寡聚（dT）-纤维素柱层析进行纯化。用含氢氧化甲基汞的琼脂糖凝胶电泳测定，该mRNA长度为1200个核苷酸。基于牛催乳素（PRL）的部分氨基酸序列，用γ-32P标记的合成寡核苷酸探针进行Northern印迹分析，证实了牛PRL mRNA，并且该mRNA能在兔网织红细胞无细胞体系中刺激35S-甲硫氨酸掺入蛋白质。牛PRL mRNA的翻译产物可被兔抗羊PRL抗抗体免疫沉淀。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和放射自显影法估计，对应于牛PRL前体的翻译产物的分子量约为25000。
