Buzina W, Braun H, Freudenschuss K, Lackner A, Schimpl K, Stammberger H
HNO-Universitätsklinik Graz, Auenbruggerplatz 26-28, A-8036 Graz, Austria.
Mycoses. 2003;46 Suppl 1:23-7.
In the last fifty years, only 22 medical cases involving the basidiomycetous fungus Schizophyllum commune were reported. In a period of three years we have examined 270 patients suffering from chronic rhinosinusitis as well as from mycoses (fungus balls) within the paranasal sinuses. Either nasal mucus or fungal concrement from the sinuses were cultured and the resulting cultures identified microscopically. In cases, where a reliable identification of the fungi was not possible, DNA was extracted for molecular examination. The internal transcribed spacer (ITS) region of the ribosomal gene-cluster was amplified with fungus specific primers and sequenced thereafter. In addition, DNA of all fungi growing with sterile white mycelium was amplified with the primer pair scom1/scom2r, which is specific for S. commune. Altogether, within a three years period S. commune was isolated in twelve patients. It can be assumed, that with the presented methods S. commune will be found much more frequently in patients suffering from diseases of the nasal sinuses.
在过去的五十年里，仅报道了22例涉及担子菌裂褶菌的医学病例。在三年时间里，我们检查了270例患有慢性鼻窦炎以及鼻窦内真菌病（真菌球）的患者。对来自鼻窦的鼻黏液或真菌凝块进行培养，并对所得培养物进行显微镜鉴定。在无法可靠鉴定真菌的情况下，提取DNA进行分子检测。用真菌特异性引物扩增核糖体基因簇的内部转录间隔区（ITS），然后进行测序。此外，用对裂褶菌特异的引物对scom1/scom2r扩增所有生长出无菌白色菌丝体的真菌的DNA。在三年时间里，总共在12例患者中分离出了裂褶菌。可以推测，采用本文所介绍的方法，在患有鼻窦疾病的患者中会更频繁地发现裂褶菌。
