Chen Y
Laboratory of Genetic Engineering Fujian Medical College, Fuzhou, China.
Chin J Biotechnol. 1992;8(3):179-86.
DA4-4 (ATCC HB57) is a shear sensitive mouse-mouse hybridoma cell, producing an IgG1 monoclonal antibody against human IgM. It was grown successfully in a perfusion propagation system consisting of a 1.5 CelliGen stirred bioreactor and a hollow fiber cartridge. CelliGen system produced low shear force and cells could grow well at high agitation of 150 rpm. The culture was maintained for 40 days and cell number reached 20 x 10(6)/ml. Maximal monoclonal antibody concentration was 4.75 mg/ml. This system produced about 1.0 g of antibody every day at its peaks.
DA4-4（美国典型培养物保藏中心HB57）是一种对剪切力敏感的小鼠-小鼠杂交瘤细胞，可产生抗人IgM的IgG1单克隆抗体。它在由1.5 CelliGen搅拌式生物反应器和中空纤维柱组成的灌注培养系统中成功生长。CelliGen系统产生的剪切力较低，细胞在150转/分钟的高搅拌速度下能够良好生长。培养持续了40天，细胞密度达到20×10⁶/毫升。单克隆抗体的最大浓度为4.75毫克/毫升。该系统在高峰期每天可产生约1.0克抗体。
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