[中空纤维灌注生物反应器中抗乙型肝炎表面抗原单克隆抗体的连续生产]

[Continuous production of anti-hepatitis B surface antigen monoclonal antibody in hollow fiber perfusion bioreactor].

作者信息

Meng M H, Chen G C, Hsieh J H, Yueh Y, Chen S C, Chang T H, Tsao D

机构信息

Development Center for Biotechnology, Taipei, Taiwan, ROC.

出版信息

Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1988 Aug;21(3):125-40.

PMID:3072154

Abstract

Hybridoma cell line H440Ac was cultivated continuously in hollow fiber perfusion bioreactor for fifty days to produce anti-hepatitis B surface antigen (anti-HBsAg) monoclonal antibody. A total of 90 mg anti-HBsAg antibody was produced in the hollow fiber bioreactor with 1000 cm2 membrane surface area. The collected production medium has an average antibody concentration of 0.45 mg/ml with a high concentration of 2.3 mg/ml. This compares with 0.016 and 1.1 mg/ml antibody concentration as collected from spinner flask and mouse ascites. The purity of the produced antibody is 0.085, 0.0028 and 0.026 mg antibody per mg of total harvested protein in the culture media produced from the hollow fiber bioreactor, spinner flasks and mouse ascites, respectively.

摘要

杂交瘤细胞系H440Ac在中空纤维灌注生物反应器中连续培养50天，以生产抗乙型肝炎表面抗原（抗-HBsAg）单克隆抗体。在膜表面积为1000 cm²的中空纤维生物反应器中总共产生了90 mg抗-HBsAg抗体。收集的生产培养基中抗体平均浓度为0.45 mg/ml，高浓度为2.3 mg/ml。相比之下，从转瓶和小鼠腹水中收集的抗体浓度分别为0.016 mg/ml和1.1 mg/ml。中空纤维生物反应器、转瓶和小鼠腹水产生的培养基中，每毫克总收获蛋白产生的抗体纯度分别为0.085 mg、0.0028 mg和0.026 mg。