LaManna J C, Griffith J K, Cordisco B R, Lin C W, Lust W D
Department of Neurology, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
Can J Physiol Pharmacol. 1992;70 Suppl:S269-77. doi: 10.1139/y92-272.
Intracellular pH can be measured quantitatively in rat brain in vivo and in vitro using spectrophotometric detection of the vital dye neutral red. This method preserves spatial information and is compatible with microhistochemistry. The intracellular pH indicated by this method is in close agreement with that indicated by 31P-NMR spectroscopy. During ischemia, intracellular acidification is correlated with tissue lactate accumulation. The spatial distribution of pH values becomes more heterogeneous as the tissue becomes more acidic. Resuscitation from total cerebral ischemia produced by cardiac arrest results in rapid intracellular realkalinization. This realkalinization is at least partially inhibited by amiloride pretreatment. Some neuronal populations, especially in the hippocampal CA1 and CA4 regions, may become more acidic during ischemia and realkalinize more slowly after reperfusion than other tissue regions. The intracellular pH of hippocampal brain slice preparations is more alkaline than expected from in vivo studies. The intracellular pH of the brain slice can be acidified to near neutrality by specific inhibitors of the sodium/hydrogen ion exchanger.
使用活体染料中性红的分光光度检测法，可在体内和体外对大鼠脑组织中的细胞内pH值进行定量测量。该方法保留了空间信息，并且与显微组织化学兼容。此方法所显示的细胞内pH值与31P-核磁共振波谱法所显示的结果高度一致。在缺血期间，细胞内酸化与组织乳酸积累相关。随着组织酸性增强，pH值的空间分布变得更加不均匀。心脏骤停导致的全脑缺血复苏后，细胞内会迅速发生再碱化。这种再碱化至少部分受到氨氯地平预处理的抑制。一些神经元群体，特别是海马CA1和CA4区域的神经元，在缺血期间可能会变得更酸，并且再灌注后再碱化的速度比其他组织区域更慢。海马脑片制剂的细胞内pH值比体内研究预期的更偏碱性。脑片的细胞内pH值可通过钠/氢离子交换器的特异性抑制剂酸化至接近中性。
