Shostak Katherina, Labunskyy Vyacheslav, Dmitrenko Vladimir, Malisheva Tatiana, Shamayev Michail, Rozumenko Vladimir, Zozulya Yuriy, Zehetner Günter, Kavsan Vadym
Institute of Molecular Biology and Genetics, 150 Zabolotnogo str., 03143 Kiev, Ukraine.
Cancer Lett. 2003 Aug 20;198(2):203-10. doi: 10.1016/s0304-3835(03)00310-0.
Public databases of the Cancer Genome Anatomy Project were used to quantify the relative gene expression levels in glioblastoma multiforme (GBM) and normal brain by Serial Analysis of Gene Expression (SAGE). Analysis revealed HC gp-39 among the genes with the most pronounced changes of expression in tumor cells. Northern hybridization confirmed the results of computer analysis and showed that enhanced expression of the HC gp-39 gene was mainly in GBMs and occasionally in anaplastic astrocytomas. Neither SAGE nor Northern analysis revealed the presence of HC gp-39 mRNA in the glioblastoma cell line, thus the detection of increased quantities of this mRNA in GBMs may be associated with activated macrophages. Since the numbers of infiltrating macrophages and small vessel density are higher in glioblastomas than in anaplastic astrocytomas or astrocytomas, the HC gp-39 gene can be used as a molecular marker in the analysis of malignant progression of astrocytic gliomas.
癌症基因组解剖计划的公共数据库被用于通过基因表达序列分析（SAGE）来量化多形性胶质母细胞瘤（GBM）和正常脑组织中的相对基因表达水平。分析显示，HC gp-39是肿瘤细胞中表达变化最为显著的基因之一。Northern杂交证实了计算机分析的结果，并表明HC gp-39基因的表达增强主要见于多形性胶质母细胞瘤，偶尔见于间变性星形细胞瘤。SAGE分析和Northern分析均未在胶质母细胞瘤细胞系中检测到HC gp-39 mRNA，因此，在多形性胶质母细胞瘤中检测到该mRNA数量增加可能与活化的巨噬细胞有关。由于多形性胶质母细胞瘤中浸润性巨噬细胞数量和小血管密度高于间变性星形细胞瘤或星形细胞瘤，因此HC gp-39基因可作为分析星形细胞胶质瘤恶性进展的分子标志物。
