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2
Detection and quantification of the red tide dinoflagellate Karenia brevis by real-time nucleic acid sequence-based amplification.基于实时核酸序列扩增技术对赤潮甲藻短裸甲藻的检测与定量分析。
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Detection and quantification of the red tide dinoflagellate Karenia brevis by real-time nucleic acid sequence-based amplification.基于实时核酸序列扩增技术对赤潮甲藻短裸甲藻的检测与定量分析。
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本文引用的文献

1
Photoautotrophy in Gymnodinium breve Davis.短裸甲藻的光自养。
Science. 1962 Sep 21;137(3534):988-90. doi: 10.1126/science.137.3534.988.
2
Extraction from natural planktonic microorganisms of DNA suitable for molecular biological studies.从天然浮游微生物中提取适合分子生物学研究的 DNA。
Appl Environ Microbiol. 1988 Jun;54(6):1426-9. doi: 10.1128/aem.54.6.1426-1429.1988.
3
Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran.海洋浮游硅藻的研究。I. 微小环藻(Hustedt),以及脆杆藻(Cleve)格兰变种
Can J Microbiol. 1962 Apr;8:229-39. doi: 10.1139/m62-029.
4
A single origin of the peridinin- and fucoxanthin-containing plastids in dinoflagellates through tertiary endosymbiosis.通过三次内共生,双鞭毛虫中含多甲藻素和岩藻黄质的质体起源单一。
Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11724-9. doi: 10.1073/pnas.172234799. Epub 2002 Aug 9.
5
Second- and third-hand chloroplasts in dinoflagellates: phylogeny of oxygen-evolving enhancer 1 (PsbO) protein reveals replacement of a nuclear-encoded plastid gene by that of a haptophyte tertiary endosymbiont.甲藻中的二手和三手叶绿体:放氧增强子1(PsbO)蛋白的系统发育揭示了一个核编码的质体基因被一个定鞭藻第三级内共生体的基因所取代。
Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9294-9. doi: 10.1073/pnas.142091799. Epub 2002 Jun 27.

海洋环境中赤潮甲藻短裸甲藻的分子检测与定量分析。

Molecular detection and quantitation of the red tide dinoflagellate Karenia brevis in the marine environment.

作者信息

Gray M, Wawrik B, Paul J, Casper E

机构信息

University of South Florida, College of Marine Science, St. Petersburg, Florida 33701, USA.

出版信息

Appl Environ Microbiol. 2003 Sep;69(9):5726-30. doi: 10.1128/AEM.69.9.5726-5730.2003.

DOI:10.1128/AEM.69.9.5726-5730.2003
PMID:12957971
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC194946/
Abstract

A real-time reverse transcription-PCR method targeting the rbcL gene was developed for the detection and quantitation of the Florida red tide organism, Karenia brevis. The assay was sensitive to less than 1 cell per reaction, did not detect rbcL from 38 nontarget taxa, and accurately quantitated K. brevis organisms in red tide samples from around Florida. These studies have resulted in a sensitive and specific method for K. brevis detection in the marine environment.

摘要

开发了一种针对rbcL基因的实时逆转录聚合酶链反应方法,用于检测和定量佛罗里达红潮生物——短裸甲藻。该检测方法对每个反应中少于1个细胞敏感,未从38个非目标分类群中检测到rbcL,并准确地定量了来自佛罗里达州各地红潮样本中的短裸甲藻生物。这些研究产生了一种用于在海洋环境中检测短裸甲藻的灵敏且特异的方法。