海洋环境中赤潮甲藻短裸甲藻的分子检测与定量分析。
Molecular detection and quantitation of the red tide dinoflagellate Karenia brevis in the marine environment.
作者信息
Gray M, Wawrik B, Paul J, Casper E
机构信息
University of South Florida, College of Marine Science, St. Petersburg, Florida 33701, USA.
出版信息
Appl Environ Microbiol. 2003 Sep;69(9):5726-30. doi: 10.1128/AEM.69.9.5726-5730.2003.
Abstract
A real-time reverse transcription-PCR method targeting the rbcL gene was developed for the detection and quantitation of the Florida red tide organism, Karenia brevis. The assay was sensitive to less than 1 cell per reaction, did not detect rbcL from 38 nontarget taxa, and accurately quantitated K. brevis organisms in red tide samples from around Florida. These studies have resulted in a sensitive and specific method for K. brevis detection in the marine environment.
摘要
开发了一种针对rbcL基因的实时逆转录聚合酶链反应方法，用于检测和定量佛罗里达红潮生物——短裸甲藻。该检测方法对每个反应中少于1个细胞敏感，未从38个非目标分类群中检测到rbcL，并准确地定量了来自佛罗里达州各地红潮样本中的短裸甲藻生物。这些研究产生了一种用于在海洋环境中检测短裸甲藻的灵敏且特异的方法。
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