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培养海马切片中长时程增强（LTP）的长期记录。

Long-term recording of LTP in cultured hippocampal slices.

作者信息

Shimono Ken, Baudry Michel, Ho Lam, Taketani Makoto, Lynch Gary

机构信息

Tensor Biosciences, Irvine, CA 92612, USA.

出版信息

Neural Plast. 2002;9(4):249-54. doi: 10.1155/NP.2002.249.

DOI:10.1155/NP.2002.249
PMID:12959154
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2565406/
Abstract

Long-term potentiation (LTP) was elicited by high frequency stimulation in hippocampal slices cultured on multi-electrode arrays. LTP lasting more than 1 h was recorded in 75% of slices, and a significant number of slices exhibited a non-decaying LTP that lasted more than 48 h. LTP induction was completely and reversibly blocked by an antagonist of the NMDA receptor, APV. Our results suggest the possibility of using chronic recording in hippocampal slices cultured on multi-electrode arrays to study the mechanisms underlying LTP maintenance and stabilization.

摘要

在多电极阵列上培养的海马切片中，通过高频刺激诱发长时程增强（LTP）。在75%的切片中记录到持续超过1小时的LTP，并且大量切片表现出持续超过48小时的非衰减LTP。NMDA受体拮抗剂APV完全且可逆地阻断了LTP的诱导。我们的结果表明，利用在多电极阵列上培养的海马切片进行长期记录来研究LTP维持和稳定的潜在机制是有可能的。

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