Zanella Rosemeire C, Brandileone Maria Cristina C, Bokermann Sérgio, Almeida Samanta C G, Valdetaro Fábio, Vitório Fábio, Moreira Maria de Fátima A, Villins Margarete, Salomão Reinaldo, Pignatari Antonio Carlos C
Laboratory of Bacteriology, Institute Adolfo Lutz, São Paulo, Brazil.
Microb Drug Resist. 2003 Fall;9(3):283-91. doi: 10.1089/107662903322286490.
We report the phenotypic and genotypic characterization of 50 VanA Enterococcus clinical isolates from infected patients and 97 isolates from colonized patients obtained during a nosocomial outbreak in a single hospital in São Paulo, Brazil during 1998. The identification of strains to the species level by conventional biochemical and phenotypic tests and by multiplex PCR assay had 100% agreement. Both E. faecalis and E. faecium were isolated from patients during this outbreak. The vanA genotype was confirmed by PCR. Antibiotic susceptibility testing showed that E. faecium isolates are generally less susceptible to antibiotics than E. faecalis. By PCR, 24 of 26 VRE strains tested carried the Tn1546 element. Pulsed-field gel electrophoresis identified five distinct patterns for E. faecalis (A, B, C, D, E) and three for E. faecium (M, N, and O). A single PFGE pattern was identified in the majority of strains of each species and does not discriminate between case and carrier isolates.
我们报告了1998年在巴西圣保罗一家医院的医院感染暴发期间，从感染患者中分离出的50株VanA肠球菌临床分离株以及从定植患者中分离出的97株分离株的表型和基因型特征。通过传统生化和表型试验以及多重PCR检测将菌株鉴定到种水平，结果具有100%的一致性。在此暴发期间，从患者中分离出了粪肠球菌和屎肠球菌。通过PCR确认了vanA基因型。抗生素敏感性试验表明，屎肠球菌分离株通常比粪肠球菌对抗生素更不敏感。通过PCR，在检测的26株VRE菌株中有24株携带Tn1546元件。脉冲场凝胶电泳确定了粪肠球菌的五种不同模式（A、B、C、D、E）和屎肠球菌的三种不同模式（M、N和O）。在每个菌种的大多数菌株中鉴定出单一的PFGE模式，且该模式无法区分病例和携带者分离株。
