Akitaya Tatsuo, Tsumoto Kanta, Yamada Ayako, Makita Naoko, Kubo Koji, Yoshikawa Kenichi
Department of Physics, Graduate School of Science, Kyoto University, and CREST, Kyoto 606-8502, Japan.
Biomacromolecules. 2003 Sep-Oct;4(5):1121-5. doi: 10.1021/bm034017w.
It is becoming clearer that genetic activity is closely associated with the intracellular energy state. However, the mechanisms of this association are still unclear. In this study, we focused on large-scale changes in the structure of DNA to examine the effect of the NTP concentration on the transcription reaction with T7 RNA polymerase and compared the results with long duplex DNA to those with a short persistent-length(1) fragment. The transcriptional activity dramatically changed only for long duplex DNA within a narrow range of NTP concentrations associated with changes in the large-scale structure of DNA. This result suggests that the energy state may play an essential role in regulating ON/OFF switching on transcriptional activity.
越来越清楚的是，基因活性与细胞内能量状态密切相关。然而，这种关联的机制仍不清楚。在本研究中，我们聚焦于DNA结构的大规模变化，以研究NTP浓度对T7 RNA聚合酶转录反应的影响，并将长双链DNA的结果与具有短持久长度(1)片段的结果进行比较。仅在与DNA大规模结构变化相关的狭窄NTP浓度范围内，长双链DNA的转录活性发生了显著变化。这一结果表明，能量状态可能在调节转录活性的开/关转换中起关键作用。
