Essman Stephanie C, Lattimer Jim, Cook James L, Turnquist Sue, Kuroki Keiichi
Department of Medicine and Surgery, University of Missouri-Columbia, 65211, USA.
J Nucl Med. 2003 Sep;44(9):1510-5.
Previous studies reported that the radiopharmaceutical (153)Sm-ethylenediaminetetramethylene phosphonate ((153)Sm-EDTMP) is an effective component of multimodality therapy for the treatment of primary bone tumors. Therefore, (153)Sm-EDTMP may prove to be an integral component of therapy for the treatment of juvenile osteosarcoma. The purpose of this study was to determine the effects of intravenous administration of (153)Sm-EDTMP on the developing physeal and articular cartilage of healthy, juvenile rabbits.
Sixteen healthy 8-wk-old male New Zealand White rabbits were assigned to 1 of 2 groups: treatment (n = 12) and control (n = 4). (153)Sm-EDTMP was administered to the treatment group at 37 MBq/kg (1 mCi/kg). The animals were sacrificed at 16 wk of age, and the physeal cartilage of multiple bones was evaluated by use of histologic, immunohistochemical, and histomorphometric analyses. The overall changes in the lengths of the radius and the tibia between control and treatment groups were calculated and compared. Measurement data were combined for each group, and means +/- SEMs were determined.
Significant differences in radial bone growth were present between the groups. Histologically, the physes of the treatment group were disrupted and chaotic in appearance. Significant differences in the immunoreactivity of type X collagen and matrix metalloproteinase-13 were seen between the groups, as these markers were positively expressed in the zone of hypertrophy of the control rabbits.
Clinically significant damage to the developing physeal cartilage may occur as a result of the intravenous administration of (153)Sm-EDTMP at the dose studied.
先前的研究报道，放射性药物钐-153-乙二胺四亚甲基膦酸盐（153Sm-EDTMP）是治疗原发性骨肿瘤的多模态疗法的有效成分。因此，153Sm-EDTMP可能被证明是治疗青少年骨肉瘤的重要治疗成分。本研究的目的是确定静脉注射153Sm-EDTMP对健康幼年兔生长中的骺软骨和关节软骨的影响。
将16只健康的8周龄雄性新西兰白兔分为2组中的1组：治疗组（n = 12）和对照组（n = 4）。以37 MBq/kg（1 mCi/kg）的剂量给治疗组注射153Sm-EDTMP。在动物16周龄时将其处死，通过组织学、免疫组织化学和组织形态计量学分析评估多块骨头的骺软骨。计算并比较对照组和治疗组之间桡骨和胫骨长度的总体变化。合并每组的测量数据，并确定均值±标准误。
两组之间桡骨生长存在显著差异。在组织学上，治疗组的骺看起来被破坏且混乱。两组之间X型胶原蛋白和基质金属蛋白酶-13的免疫反应性存在显著差异，因为这些标志物在对照兔的肥大带中呈阳性表达。
在所研究的剂量下，静脉注射153Sm-EDTMP可能会对发育中的骺软骨造成具有临床意义的损伤。
