Miyazaki Satomi, Tsuda Hiroshi, Sakai Masatoshi, Hori Shinichi, Sasaki Yasushi, Futatani Takeshi, Miyawaki Toshio, Saito Shigeru
Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical University, Japan.
J Leukoc Biol. 2003 Oct;74(4):514-22. doi: 10.1189/jlb.1102566. Epub 2003 Jul 15.
Dendritic cells (DCs) are specialized antigen-presenting cells required for the priming and activation of T cells and promote the differentiation of naïve CD4+ T cells toward the T helper cell type 1 (Th1) or Th2 phenotype. Here, we describe the characterization of CD45+CD3-CD14-CD16-CD19-CD20-CD56-HLA-DRbright DCs from early human pregnancy decidua by flow cytometry. The percentage of DCs to mononuclear cells (leukocytes) in the decidua was significantly higher than that in the peripheral blood. Moreover, decidual DCs expressed costimulatory molecules such as CD80 and CD86 and a mature marker such as CD83 on their surface. The percentage of CD11c+CD123- myeloid DCs in the decidua was significantly higher than that in the peripheral blood. Conversely, the ratio of CD11c-CD123+ lymphoid DCs in the decidua was significantly lower than that in the peripheral blood. The number of interleukin (IL)-12-producing cells in the total DC population and the myeloid DCs in the decidua was significantly lower than that in the peripheral blood. IL-12 secretion by activated decidual myeloid DCs was significantly lower than that by peripheral DCs. Naïve CD4+ T cells primed with decidual myeloid DCs led to a higher percentage of Th2 cells in comparison with that with peripheral myeloid DCs. This finding was abolished by exogenous IL-12 administration with decidual myeloid DCs. Thus, the DCs in the decidua could regulate the Th1/Th2 balance to maintain a Th2-dominant state, leading to maintenance of pregnancy.
树突状细胞（DCs）是启动和激活T细胞所必需的专业抗原呈递细胞，并促进初始CD4+ T细胞向1型辅助性T细胞（Th1）或Th2表型分化。在此，我们通过流式细胞术描述了来自早期人类妊娠蜕膜的CD45+CD3-CD14-CD16-CD19-CD20-CD56-HLA-DRbright DCs的特征。蜕膜中DCs占单核细胞（白细胞）的百分比显著高于外周血。此外，蜕膜DCs在其表面表达共刺激分子如CD80和CD86以及成熟标志物如CD83。蜕膜中CD11c+CD123-髓样DCs的百分比显著高于外周血。相反，蜕膜中CD11c-CD123+淋巴样DCs的比例显著低于外周血。蜕膜中总DC群体和髓样DCs中产生白细胞介素（IL）-12的细胞数量显著低于外周血。激活的蜕膜髓样DCs分泌的IL-12显著低于外周DCs。与外周髓样DCs相比，用蜕膜髓样DCs启动的初始CD4+ T细胞导致Th2细胞的百分比更高。用蜕膜髓样DCs外源性给予IL-12可消除这一发现。因此，蜕膜中的DCs可以调节Th1/Th2平衡以维持Th2主导状态，从而维持妊娠。
