Xing H Rosie, Cordon-Cardo Carlos, Deng Xinzhu, Tong William, Campodonico Luis, Fuks Zvi, Kolesnick Richard
Laboratory of Signal Transduction, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA.
Nat Med. 2003 Oct;9(10):1266-8. doi: 10.1038/nm927. Epub 2003 Sep 7.
Inhibition of the kinase suppressor of ras-1 (KSR1) gene by continuous infusion of phosphorothioate antisense oligonucleotides (ODNs) prevented growth of K-Ras-dependent human PANC-1 pancreatic and A549 non-small-cell lung carcinoma xenografts in nude mice, effected regression of established PANC-1 tumors and inhibited A549 lung metastases, all without apparent toxicity. These studies suggest KSR1 antisense ODNs as a treatment for Ras-dependent human malignancies, in particular pancreatic cancer, which lacks effective curative therapy.
通过持续输注硫代磷酸酯反义寡核苷酸（ODN）抑制ras-1激酶抑制因子（KSR1）基因，可阻止依赖K-Ras的人胰腺癌细胞PANC-1和非小细胞肺癌细胞A549在裸鼠体内异种移植瘤的生长，使已形成的PANC-1肿瘤消退，并抑制A549肺转移，且均无明显毒性。这些研究表明，KSR1反义ODN可作为治疗依赖Ras的人类恶性肿瘤的方法，尤其是胰腺癌，因为目前胰腺癌缺乏有效的治愈性疗法。
