Halford Colin, Samson Sue E, Kwan Chiu Yin, Grover Ashok K
Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
Mol Cell Biochem. 2003 Aug;250(1-2):197-201. doi: 10.1023/a:1024942526849.
Here, we report that the smooth muscle and endothelium of the pig coronary artery differ in the profiles of energy metabolism nucleotides. ATP levels in the freshly isolated smooth muscle (1490 +/- 93, all the values are in pmol/mg protein) were significantly greater than in the endothelium (418 +/- 68). In contrast, endothelium contained higher levels of NADH (328 +/- 21), NAD+ (1210 -/+ 28), NADPH (87 +/- 2), and NADP+ (77 +/- 4) than smooth muscle (17 +/- 2, 96 +/- 14, 7 +/- 1, and 8 +/- 1, respectively). However, smooth muscle and endothelium do not differ from each other in the ratios of NADH/NAD+ or NADPH/NADP+. Cells cultured from smooth muscle and endothelium contained less ATP (93 +/- 2, 141 +/- 6) and had lower ratios of NADH/ NAD+ than the freshly isolated tissues but the NADPH/NADP+ ratios remained similar. We conclude that (a) freshly isolated smooth muscle and endothelium differ in their profiles of the energy metabolism nucleotides, and (b) culturing the cells alters the profile.
在此，我们报告猪冠状动脉的平滑肌和内皮在能量代谢核苷酸谱方面存在差异。新鲜分离的平滑肌中的ATP水平（1490±93，所有数值单位为pmol/mg蛋白质）显著高于内皮（418±68）。相比之下，内皮中NADH（328±21）、NAD⁺（1210±28）、NADPH（87±2）和NADP⁺（77±4）的水平高于平滑肌（分别为17±2、96±14、7±1和8±1）。然而，平滑肌和内皮在NADH/NAD⁺或NADPH/NADP⁺的比率上彼此无差异。从平滑肌和内皮培养而来的细胞所含ATP较少（93±2、141±6），且NADH/NAD⁺的比率低于新鲜分离的组织，但NADPH/NADP⁺的比率保持相似。我们得出结论：（a）新鲜分离的平滑肌和内皮在能量代谢核苷酸谱方面存在差异，且（b）细胞培养会改变该谱。
