Solé M, Cano J, Rodríguez-Tudela J L, Pontón J, Sutton D A, Perrie R, Gené J, Rodríguez V, Guarro J
Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Institut d'Estudis Avançats, Universitat Rovira i Virgili, 43201-Reus, Tarragona, Spain.
Med Mycol. 2003 Aug;41(4):293-300. doi: 10.1080/13693780310001600813.
Invasive infections by Scedosporium prolificans have increased alarmingly in recent years, mainly in immunosuppressed patients. The epidemiology, pathogenesis and the natural habitat of this pathogen are practically unknown. Isolates of S. prolificans were distinguished from one another by inter-simple-sequence-repeat (ISSR) fingerprinting, a technique based on the high degree of polymorphism of the multisatellite genetic markers used. This technique was found useful for typing 84 isolates of S. prolificans from different countries and sources. The assemblage of S. prolificans isolates tested was extremely diverse, with 35 genotypes present. Several patients were found to have been infected or colonized by more than one strain. Overall, this technique facilitates the epidemiological study of S. prolificans infection.
近年来，多育赛多孢菌引起的侵袭性感染急剧增加，主要发生在免疫抑制患者中。这种病原体的流行病学、发病机制和自然栖息地几乎不为人知。多育赛多孢菌的分离株通过简单序列重复区间（ISSR）指纹分析相互区分，该技术基于所使用的多卫星遗传标记的高度多态性。结果发现，这项技术可用于对来自不同国家和来源的84株多育赛多孢菌进行分型。所测试的多育赛多孢菌分离株组合极为多样，共有35种基因型。发现有几名患者感染或定植了不止一种菌株。总体而言，这项技术有助于多育赛多孢菌感染的流行病学研究。
