Harun Azian, Perdomo Haybrig, Gilgado Felix, Chen Sharon C A, Cano Josep, Guarro Josep, Meyer Wieland
Molecular Mycology Research Laboratory, University of Sydney, Western Clinical School at Westmead Hospital, Westmead Millennium Institute, Sydney, Australia.
Med Mycol. 2009 Jun;47(4):406-14. doi: 10.1080/13693780802510240. Epub 2008 Dec 12.
Scedosporium species are increasingly encountered opportunistic fungal pathogens not only in immunocompromised patients but are also significant primary pathogens in immunocompetent individuals. The environmental reservoir of these fungi is uncertain and the epidemiology and mode of transmission are not well-defined. Conventional phenotypic methods are of limited use for epidemiological purposes since they are insensitive and inadequately discriminatory. Molecular techniques not only enable accurate phylogenetic delineation of species but also provide the means for rapid, reliable genotyping of strains for epidemiological and population genetic studies. This review discusses the methods that have been applied for genotyping of these increasingly important pathogens.
赛多孢属真菌不仅在免疫功能低下的患者中越来越多地成为机会性真菌病原体，在免疫功能正常的个体中也是重要的原发性病原体。这些真菌的环境宿主尚不确定，其流行病学和传播方式也未明确界定。传统的表型方法在流行病学研究中的用途有限，因为它们不敏感且鉴别能力不足。分子技术不仅能对物种进行准确的系统发育划分，还为菌株的快速、可靠基因分型提供了手段，用于流行病学和群体遗传学研究。本文综述了已应用于这些日益重要病原体基因分型的方法。
