Anding Allyson L, Reiss Tanika, Germain Glen S
Department of Molecular Pharmacology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
In Vitro Cell Dev Biol Anim. 2003 Jul-Aug;39(7):273-4. doi: 10.1290/1543-706X(2003)039<0273:AOTVNT>2.0.CO;2.
Using primers for the MCT118, YNZ22, and COL2A1 loci in polymerase chain reaction analysis we could distinguish among the approximately 20 cell lines routinely maintained in our laboratory. We also demonstrated that the cell line NB-1691 (a neuroblastoma) and its xenograft had an identical number of repeats at two loci. Rh30 (a rhabdomyosarcoma) made resistant to rapamycin was identical to its parent line and to a subline that had reverted to sensitivity after it was cultured without rapamycin in the medium.
在聚合酶链反应分析中，使用针对MCT118、YNZ22和COL2A1基因座的引物，我们能够区分在我们实验室常规培养的约20种细胞系。我们还证明，细胞系NB - 1691（一种神经母细胞瘤）及其异种移植物在两个基因座具有相同数量的重复序列。对雷帕霉素产生抗性的Rh30（一种横纹肌肉瘤）与其亲本系以及在无雷帕霉素培养基中培养后恢复敏感性的亚系相同。
