Bukowska Bozena
Department of Biophysics of Environmental Pollution, University of Lódz, Banacha 12/16, 90-237 Lódz, Poland.
Comp Biochem Physiol C Toxicol Pharmacol. 2003 Aug;135(4):435-41. doi: 10.1016/s1532-0456(03)00151-0.
The effects of in vitro exposure of human erythrocytes to different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and its metabolite 2,4-dichlorophenol (2,4-DCP) were studied. The activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the level of reduced glutathione (GSH) were determined. The activity of erythrocyte superoxide dismutase SOD decreased with increasing dose of 2,4-D and 2,4-DCP, while glutathione peroxidase activity increased. 2,4-D (500 ppm) decreased the level of reduced glutathione in erythrocytes by 18% and 2,4-DCP (250 ppm) by 32%, respectively, in comparison with the controls. These results lead to the conclusion that in vitro administration of herbicide-2,4-D and its metabolite 2,4-DCP causes a decrease in the level of reduced glutathione in erythrocytes and significant changes in antioxidant enzyme activities. Comparison of the toxicity of 2,4-D and 2,4-DCP revealed that the most prominent changes occurred in human erythrocytes incubated with 2,4-DCP.
研究了人体红细胞在体外暴露于不同浓度的2,4-二氯苯氧乙酸（2,4-D）及其代谢产物2,4-二氯苯酚（2,4-DCP）的影响。测定了超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活性以及还原型谷胱甘肽（GSH）的水平。红细胞超氧化物歧化酶SOD的活性随2,4-D和2,4-DCP剂量的增加而降低，而谷胱甘肽过氧化物酶的活性增加。与对照组相比，2,4-D（500 ppm）使红细胞中还原型谷胱甘肽水平分别降低了18%，2,4-DCP（250 ppm）使还原型谷胱甘肽水平降低了32%。这些结果得出结论，体外给予除草剂2,4-D及其代谢产物2,4-DCP会导致红细胞中还原型谷胱甘肽水平降低以及抗氧化酶活性发生显著变化。2,4-D和2,4-DCP毒性的比较表明，与2,4-DCP孵育的人体红细胞中出现的变化最为显著。
