Guerriero Chiara, Zoccatelli Gianni, Stefani Elisabetta, Sartoris Silvia, Cestari Tiziana, Riviera Anna Pia, Tridente Giuseppe, Andrighetto Giancarlo, Chignola Roberto
Dipartimento di Patologia, Università di Verona, c/o Policlinico G.B. Rossi, I-37134 Verona, Italy.
J Neuroimmunol. 2003 Aug;141(1-2):83-9. doi: 10.1016/s0165-5728(03)00226-1.
A previously isolated and characterized IgM monoclonal antibody (mAb 1H6.2) specific to myelin basic protein (MBP) and to MBP epitopes expressed by nonneural cells was used to immunoprecipitate and investigate the expression of MBP epitopes by human T cells. Peripheral T lymphocytes secreted MBP epitopes, and secretion increased in time after mitogen stimulation. Conversely, thymocytes secreted these proteins independently on mitogen stimulation. Specific antibody reactivity (primarily due to IgG3) towards immunoprecipitated MBP epitopes was found in all tested sera from healthy donors and from multiple sclerosis patients as well as in sera from normal human cord blood. Collectively, these data provide insights into the immunological mechanisms leading to central and peripheral tolerance to MBP products.
一种先前分离并鉴定的针对髓鞘碱性蛋白（MBP）以及非神经细胞表达的MBP表位的IgM单克隆抗体（单克隆抗体1H6.2）被用于免疫沉淀并研究人T细胞中MBP表位的表达。外周T淋巴细胞分泌MBP表位，且在有丝分裂原刺激后分泌量随时间增加。相反，胸腺细胞在有丝分裂原刺激下独立分泌这些蛋白。在来自健康供体、多发性硬化症患者的所有测试血清以及正常人脐带血血清中均发现了针对免疫沉淀的MBP表位的特异性抗体反应性（主要归因于IgG3）。总体而言，这些数据为导致对MBP产物产生中枢和外周耐受性的免疫机制提供了见解。
