Prioult Guénolée, Fliss Ismail, Pecquet Sophie
Dairy Research Centre STELA, Département des Sciences des Aliments et de Nutrition, Université Laval, Québec, Quebec G1K 7P4, Canada.
Clin Diagn Lab Immunol. 2003 Sep;10(5):787-92. doi: 10.1128/cdli.10.5.787-792.2003.
In this study, the effect of Lactobacillus paracasei (NCC 2461), Lactobacillus johnsonii (NCC 533) and Bifidobacterium lactis Bb12 (NCC 362) on the induction and maintenance of oral tolerance to bovine beta-lactoglobulin (BLG) was investigated in mice. Germfree mice were monocolonized with one of the three strains before oral administration of whey protein to induce tolerance. Mice were then injected with BLG and sacrificed 28 or 50 days after whey protein feeding for humoral and cellular response measurement. Conventional and germfree mice were used as controls. Both humoral and cellular responses were better suppressed in conventional mice than in germfree and monoassociated mice throughout the experiment and better suppressed in L. paracasei-associated mice than in mice colonized with B. lactis or L. johnsonii. The latter two mono-associations suppressed humoral responses only partially and cellular responses not at all. This study provides evidence that probiotics modulate the oral tolerance response to BLG in mice. The mono-colonization effect is strain-dependant, the best result having been obtained with L. paracasei.
在本研究中，研究了副干酪乳杆菌（NCC 2461）、约氏乳杆菌（NCC 533）和双歧杆菌Bb12（NCC 362）对小鼠诱导和维持对牛β-乳球蛋白（BLG）口服耐受性的影响。在口服乳清蛋白诱导耐受性之前，将无菌小鼠用这三种菌株之一进行单菌定植。然后给小鼠注射BLG，并在喂食乳清蛋白后28或50天处死，以测量体液和细胞反应。常规小鼠和无菌小鼠用作对照。在整个实验过程中，常规小鼠的体液和细胞反应比无菌小鼠和单菌定植小鼠受到更好的抑制，副干酪乳杆菌定植小鼠的反应比双歧杆菌或约氏乳杆菌定植小鼠受到更好的抑制。后两种单菌定植仅部分抑制体液反应，对细胞反应则完全没有抑制作用。本研究提供了证据表明益生菌可调节小鼠对BLG的口服耐受性反应。单菌定植效应具有菌株依赖性，副干酪乳杆菌取得了最佳结果。
