使用单克隆抗体对嗜吞噬细胞无形体分离株进行血清分型。
Serotyping isolates of Anaplasma phagocytophilum by using monoclonal antibodies.
作者信息
Inokuma Hisashi, Brouqui Philippe, Dumler J Stephen, Raoult Didier
机构信息
Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, 753-8515 Yamaguchi, Japan.
出版信息
Clin Diagn Lab Immunol. 2003 Sep;10(5):969-72. doi: 10.1128/cdli.10.5.969-972.2003.
Abstract
Ten mouse monoclonal antibodies (MAbs) that react with Anaplasma phagocytophilum (the human granulocytic ehrlichiosis agent) Webster isolates were developed. Seven different isolates of A. phagocytophilum were subtyped with these MAbs. Western blot analysis revealed that these MAbs reacted mainly with 41- to 46-kDa Msp2 proteins. Six MAbs reacted with all isolates. Four other MAbs reacted with human isolates from Wisconsin, but not with human isolates from New York or with animal isolates. Three different serotypes were identified. These features may lead to the development of other specific MAbs in order to provide tools for antigenic characterization of human isolates of A. phagocytophilum.
摘要
开发出了10种与嗜吞噬细胞无形体（人类粒细胞埃立克体病病原体）韦伯斯特分离株发生反应的小鼠单克隆抗体（MAb）。用这些单克隆抗体对7种不同的嗜吞噬细胞无形体分离株进行了亚型分类。蛋白质印迹分析表明，这些单克隆抗体主要与41至46 kDa的Msp2蛋白发生反应。6种单克隆抗体与所有分离株发生反应。另外4种单克隆抗体与来自威斯康星州的人类分离株发生反应，但不与来自纽约的人类分离株或动物分离株发生反应。鉴定出了3种不同的血清型。这些特征可能会促使开发其他特异性单克隆抗体，以便为嗜吞噬细胞无形体人类分离株的抗原特性鉴定提供工具。
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