Fisher R S, Boylan M K, Xie Y
Developmental and Molecular Neuroscience Group, Mental Retardation Research Center, School of Medicine, The University of California at Los Angeles, Los Angeles, Calif 90024, USA.
Dev Neurosci. 2003 Mar-Aug;25(2-4):127-38. doi: 10.1159/000072262.
Developmental patterns of expression and localization of tachykinins in feline neocortex were determined by qualitative immunohistochemical means. Three observations were obtained. (1) By midgestation, tachykinins were progressively accumulated in an infrequent (<1%) population of interneurons (sparse dendritic spines) settled mainly in superficial and deep sites. (2) Tachykinins were in a sparse axonal innervation showing horizontal elaboration in layers I and VI and vertical elaboration within the intervening layers (II-V) of true cortical plate. (3) Tachykinin innervation of the capillary beds arose in conjunction with tachykinin interneurons instead of extending from basal cerebral or meningeal vasculature. These patterns indicate that tachykinin local circuit neurons of feline neocortex are derived, at least in part, from early-generated neocortical preplate neurons that initiate tachykinin expression after they settle into the marginal zone of primitive neocortex. In addition to their roles in peptidergic modulation of synaptic connectivity in neocortex, this innervation may participate in trophic developmental interactions leading to the establishment of neocortical vasculature.
通过定性免疫组织化学方法确定了速激肽在猫新皮质中的表达和定位的发育模式。获得了三个观察结果。（1）到妊娠中期，速激肽逐渐在主要位于浅层和深层的少量（<1%）中间神经元（稀疏树突棘）群体中积累。（2）速激肽存在于稀疏的轴突支配中，在I层和VI层呈水平分布，在真正皮质板的中间层（II - V层）呈垂直分布。（3）毛细血管床的速激肽支配与速激肽中间神经元一起出现，而不是从基底脑或脑膜血管延伸而来。这些模式表明，猫新皮质的速激肽局部回路神经元至少部分源自早期生成的新皮质前板神经元，这些神经元在定居到原始新皮质的边缘区后开始表达速激肽。除了它们在新皮质突触连接的肽能调节中的作用外，这种支配可能参与导致新皮质血管系统建立的营养性发育相互作用。
