Liu Hsin-Fu, Teng Ching-Wen, Fukuda Yoshihide, Nakano Isao, Hayashi Kazuhiko, Takamatsu Junki, Goubau Patrick, Toyoda Hidenori
Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan.
J Med Virol. 2003 Nov;71(3):385-90. doi: 10.1002/jmv.10491.
GB virus C (GBV-C) or hepatitis G virus (HGV) has been transmitted to Japanese patients with hemophilia through the frequent use of unheated blood products. Sequence analysis showed that most of the viruses isolated from these patients belonged to GBV-C/HGV genotype 1, which is usually found in persons from Africa. This may point to the origin of this virus in Japanese patients with hemophilia. The phylogeny of 11 GBV-C/HGV isolates from Japanese patients with hemophilia was investigated by a detailed analysis with a fragment spanning from the 5' non-coding region to part of the E1 gene. Except for one that belonged to the genotype 3 cluster, all isolates were GBV-C/HGV type 1. Five main clades exist within the GBV-C/HGV genotype 1 sequences. These isolates are grouped in 2 defined clades. Three of the isolates are clustered in subtype 1c clade whereas the other 7 strains formed a new statistically well-supported monophyletic group (named subtype 1e). Our results suggest that GBV-C/HGV type 1 can at present be classified into at least 5 clades and in this group a majority of Japanese patients with hemophilia was infected with a GBV-C/HGV of a unique and newly described subtype within genotype 1.
丙型肝炎病毒（GBV-C）或庚型肝炎病毒（HGV）通过频繁使用未加热的血液制品传播给了日本血友病患者。序列分析表明，从这些患者中分离出的大多数病毒属于GBV-C/HGV基因型1，该基因型通常在非洲人中发现。这可能指向这种病毒在日本血友病患者中的起源。通过对一个从5'非编码区到E1基因部分的片段进行详细分析，研究了11株来自日本血友病患者的GBV-C/HGV分离株的系统发育。除了一株属于基因型3簇外，所有分离株均为GBV-C/HGV 1型。GBV-C/HGV基因型1序列内存在五个主要分支。这些分离株分为两个明确的分支。其中三株分离株聚集在1c亚型分支中，而其他7株形成了一个新的、统计学上有充分支持的单系群（命名为1e亚型）。我们的结果表明，目前GBV-C/HGV 1型可至少分为5个分支，在这一组中，大多数日本血友病患者感染了基因型1内一种独特的、新描述的GBV-C/HGV亚型。
