携带dsz和hcu基因的重组恶臭假单胞菌能够使正十四烷中的二苯并噻吩脱硫。

Recombinant Pseudomonas putida carrying both the dsz and hcu genes can desulfurize dibenzothiophene in n-tetradecane.

作者信息

Noda Ken-ichi, Watanabe Kimiko, Maruhashi Kenji

机构信息

Bio-Refining Process Laboratory Technical Cooperation Department, Japan Cooperation Center, Petroleum, 58F Sun-shine 60 3-1-1 Ikebukuro, Toshima-ku, Tokyo 170-6058, Japan.

出版信息

Biotechnol Lett. 2003 Jul;25(14):1147-50. doi: 10.1023/a:1024521313249.

DOI:10.1023/a:1024521313249

PMID:12967002

Abstract

Pseudomonas putida IFO13696, a recombinant strain with dsz desulfurization genes, desulfurized dibenzothiophene (DBT) in water but not in n-tetradecane. By introducing into this recombinant strain the hcuABC genes that take part in the uptake of DBT in the oil phase into the cell, 82% of 1 mM DBT in n-tetradecane was degraded in 24 h by resting cells. The products of hcuABC genes thus acted in the uptake of DBT in n-tetradecane into the cells and were effective in desulfurization of DBT in the hydrocarbon phase.

摘要

恶臭假单胞菌IFO13696是一种带有dsz脱硫基因的重组菌株，它能在水中对二苯并噻吩（DBT）进行脱硫，但在正十四烷中则不能。通过将参与油相中DBT摄取进入细胞的hcuABC基因导入该重组菌株，静止细胞在24小时内将正十四烷中1 mM DBT的82%降解。因此，hcuABC基因的产物在将正十四烷中的DBT摄取到细胞中发挥作用，并且在烃相中对DBT脱硫有效。