黄曲霉基因组克隆pAF28中的部分类逆转座子DNA序列
Partial retrotransposon-like DNA sequence in the genomic clone of Aspergillus flavus, pAF28.
作者信息
Okubara Patricia A, Tibbot Brian K, Tarun Alice S, McAlpin Cesaria E, Hua Sui-Sheng T
机构信息
USDA, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.
出版信息
Mycol Res. 2003 Jul;107(Pt 7):841-6. doi: 10.1017/s0953756203008116.
A genomic clone of the aflatoxin-producing fungus Aspergillus flavus, designated pAF28, has been used as a probe for Southern blot fingerprinting of fungal strains. A large number of A. flavus strains isolated from corn fields and tree-nut orchards can be distinguished because the DNA fingerprint patterns are highly polymorphic. We have completed the sequencing of a 6355 bp insert in pAF28. The sequence features motifs and open reading frames characteristic of transposable elements of the gypsy class. We have named this new element AfRTL-1, for A. flavus retrotransposon-like DNA.
一种产生黄曲霉毒素的真菌——黄曲霉（Aspergillus flavus）的基因组克隆，命名为pAF28，已被用作真菌菌株Southern印迹指纹图谱分析的探针。从玉米田和坚果园分离出的大量黄曲霉菌株能够被区分开来，因为DNA指纹图谱具有高度多态性。我们已经完成了pAF28中一个6355 bp插入片段的测序。该序列具有吉普赛类转座元件特有的基序和开放阅读框。我们将这个新元件命名为AfRTL-1，即黄曲霉类反转录转座子样DNA。
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