Papoucheva Ekaterina, Proviz Valentina, Lambkin Christine, Goddeeris Boudewijn, Blinov Alexander
Institute of Cytology and Genetics, Novosibirsk, Russia.
Mol Phylogenet Evol. 2003 Oct;29(1):120-5. doi: 10.1016/s1055-7903(03)00075-7.
Fragments of two mitochondrial genes, cytochrome b (CytB) and Cytochrome c oxidase subunit I (COI) have been used as phylogenetic markers in Sergentia (Chironomidae, Diptera). The concatenated (1241 bp) sequences from both genes were used to infer the phylogenetic relationships among seven Sergentia species. Five of the species belong to the endemic fauna of Lake Baikal. Alignments of the nucleotide sequences were used for the construction of trees using Neighbor-Joining and maximum parsimony methods. Both methods yielded similar results. Monophyly of both Sergentia and the Baikalian endemic species was well supported. The date of origin of the endemic group of Sergentia was estimated as 25.7 MYA which closely coincides with the start of geological changes in the Baikal area. A cytological tree, based on 12 chromosomal characteristics, for the same set of Sergentia species showed a great similarity to the molecular phylogeny.
两个线粒体基因片段，即细胞色素b（CytB）和细胞色素c氧化酶亚基I（COI），已被用作摇蚊科（双翅目）塞尔根摇蚊属的系统发育标记。来自这两个基因的串联序列（1241 bp）被用于推断7种塞尔根摇蚊属物种之间的系统发育关系。其中5个物种属于贝加尔湖的特有动物群。核苷酸序列比对被用于使用邻接法和最大简约法构建树状图。两种方法得出了相似的结果。塞尔根摇蚊属和贝加尔湖特有物种的单系性都得到了有力支持。塞尔根摇蚊属特有类群的起源时间估计为2570万年前，这与贝加尔湖地区地质变化的开始时间密切吻合。基于12个染色体特征构建的同一组塞尔根摇蚊属物种的细胞学树状图与分子系统发育显示出高度相似性。
