各种水溶性螯合剂分子在脂质体介导的巨噬细胞“自杀”技术中的功效。
Efficacy of various water-soluble chelator molecules in the liposome-mediated macrophage "suicide" technique.
作者信息
van Rooijen N, Poppema A
机构信息
Department of Cell Biology, Faculty of Medicine, Free University, Amsterdam, The Netherlands.
出版信息
J Pharmacol Toxicol Methods. 1992 Dec;28(4):217-21. doi: 10.1016/1056-8719(92)90007-n.
In earlier studies, we described a technique for the "in vivo" depletion of macrophages, using liposome-encapsulated dichloromethylene bisphosphonate (C12MBP). The aim of the present study was to compare the effects on macrophages of various other liposome-delivered Ca2+ chelators: EDTA, DTPA, and EGTA. It appeared that both EDTA and DTPA as well as Ca(2+)-and Cu(2+)-EDTA complexes were also effective in the elimination of splenic macrophages when encapsulated in liposomes and intravenously injected. EGTA, a well-known and frequently used Ca2+ chelator, had no effect.
在早期研究中，我们描述了一种使用脂质体包裹的二氯亚甲基二膦酸盐（C12MBP）“体内”清除巨噬细胞的技术。本研究的目的是比较其他各种脂质体递送的Ca2+螯合剂（乙二胺四乙酸（EDTA）、二乙三胺五乙酸（DTPA）和乙二醇双四乙酸（EGTA））对巨噬细胞的影响。结果显示，当EDTA、DTPA以及Ca(2 +)-和Cu(2 +)-EDTA复合物包裹在脂质体中并静脉注射时，它们在清除脾脏巨噬细胞方面同样有效。EGTA是一种广为人知且常用的Ca2+螯合剂，却没有效果。
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