Charlton B R, Bickford A A, Chin R P, Walker R L
California Veterinary Diagnostic Laboratory System, Turlock Branch, School of Veterinary Medicine, University of California-Davis, P.O. Box 1522, Turlock, CA 95381, USA.
J Vet Diagn Invest. 1999 Sep;11(5):408-15. doi: 10.1177/104063879901100504.
Randomly amplified polymorphic DNA (RAPD) analysis was used to investigate the molecular epidemiology of 26 Mycoplasma gallisepticum (MG) isolates obtained from turkeys located in the central valley of California. The MG isolates were recovered from 5 different companies and 13 ranches. Each company had unique MG strains. No evidence of spread of MG between companies was detected. RAPD analysis of MG isolates within a ranch during an outbreak revealed only a single strain involved in each outbreak. RAPD analysis identified an isolate from 1 ranch with a banding pattern identical to that of the 6/85 vaccine strain, which had been used on that particular ranch. Similar RAPD banding patterns of isolates from different ranches within the same company suggested horizontal spread of MG between ranches. The use of 2 primer sets in RAPD analysis was critical to prevent misinterpretation of relationships between different isolates.
随机扩增多态性DNA（RAPD）分析被用于调查从加利福尼亚中央山谷火鸡中分离得到的26株鸡毒支原体（MG）的分子流行病学。这些MG分离株来自5家不同公司和13个养殖场。每家公司都有独特的MG菌株。未检测到MG在公司间传播的证据。在一次疫情期间对一个养殖场内的MG分离株进行RAPD分析显示，每次疫情仅涉及单一菌株。RAPD分析鉴定出一个来自某养殖场的分离株，其条带模式与该特定养殖场使用过的6/85疫苗株相同。同一公司内不同养殖场分离株相似的RAPD条带模式表明MG在养殖场间存在水平传播。在RAPD分析中使用2组引物对于防止不同分离株间关系的错误解读至关重要。
