Perna A M, Borgioli A, Bonacchi M, Varini C, Vaccari G
Divisione di Cardiochirurgia, Policlinico di Careggi, Firenze.
Cardiologia. 1992 Oct;37(10):721-4.
This research represents the first part of a project finalized to prolong the ischemic time of the explanted heart. Our experimental model utilizes rat hearts heterotopically transplanted into the abdomen of a recipient: a denervated heart but perfused by blood as to permit a good evaluation of the reperfusion damage. We used 3 different types of cardioplegic solutions to achieve cardiac arrest: the first of the intracellular fluid type, the second of the extracellular type and the third one as a standard cardioplegic solution supplemented by Ca++ antagonist. Cold saline 4 degrees C was used as control. Histological patterns, birefringence index and intracellular content of high energy phosphates were evaluated. Specimens were taken after 1 hour of ischemic time and after 1 hour of reperfusion. Early results show that administration of cardioplegic solution maintains birefringence index and preserve high energy phosphates: among the 3 different types of solution the intracellular type seems to achieve better results although the number of experiments is too small to show any statistical significance. These data show that this model is appropriate to test cardioplegic solutions and their effectiveness.
本研究是一个旨在延长离体心脏缺血时间的项目的第一部分。我们的实验模型采用将大鼠心脏异位移植到受体腹部：心脏去神经支配，但由血液灌注，以便对再灌注损伤进行良好评估。我们使用3种不同类型的心脏停搏液来实现心脏停搏：第一种是细胞内液型，第二种是细胞外液型，第三种是补充了钙离子拮抗剂的标准心脏停搏液。4℃冷盐水用作对照。评估了组织学模式、双折射指数和高能磷酸盐的细胞内含量。在缺血1小时后和再灌注1小时后采集标本。早期结果表明，给予心脏停搏液可维持双折射指数并保留高能磷酸盐：在3种不同类型的溶液中，细胞内液型似乎取得了更好的结果，尽管实验数量太少，无法显示任何统计学意义。这些数据表明，该模型适用于测试心脏停搏液及其有效性。
