Nguyen T K, Martínková L, Seichert L, Machek F
Department of Biotechnology, Czechoslovak Academy of Sciences, Prague.
Folia Microbiol (Praha). 1992;37(6):433-41. doi: 10.1007/BF02899902.
By using an appropriate ratio of carbon source to mineral components, we obtained comparable citrate yields in media containing different concentrations of glucose. The enzyme system of inoculum passed on gradually from "growth" state to "production" state during the mould growth. In the starch medium, the critical factors of citric acid production are the aeration efficiency of the medium and the amylase formation of the strain. The air interruption exhibited a prolonged inhibition of the production rate but not of the citrate yield in glucose medium while those parameters in starch medium containing excessive urea were briefly but severely inhibited. After being affected by these unfavorable conditions, the production activity of Aspergillus niger could be restored by applying an appropriate fermentation process.
通过使用合适的碳源与矿物质成分比例，我们在含有不同浓度葡萄糖的培养基中获得了相当的柠檬酸盐产量。在霉菌生长过程中，接种物的酶系统逐渐从“生长”状态转变为“生产”状态。在淀粉培养基中，柠檬酸生产的关键因素是培养基的通气效率和菌株淀粉酶的形成。在葡萄糖培养基中，空气中断对生产率有长时间的抑制作用，但对柠檬酸盐产量没有影响，而在含有过量尿素的淀粉培养基中，这些参数受到短暂但严重的抑制。在受到这些不利条件影响后，通过采用适当的发酵工艺，黑曲霉的生产活性可以恢复。
