从肉类中分离出的液化沙雷氏菌中存在耶尔森菌高致病性岛（HPI）。

The Yersinia HPI is present in Serratia liquefaciens isolated from meat.

作者信息

Olsson C, Olofsson T, Ahrné S, Molin G

机构信息

Department of Food Technology, Lund University, Lund, Sweden.

出版信息

Lett Appl Microbiol. 2003;37(4):275-80. doi: 10.1046/j.1472-765x.2003.01387.x.

DOI:10.1046/j.1472-765x.2003.01387.x

PMID:12969488

Abstract

AIMS

The aim of the study was to screen the Enterobacteriaceae flora of meat for the presence of bacteria harbouring the Yersinia high-pathogenicity island (HPI).

METHODS AND RESULTS

Bacteria from 29 meat and 29 liver samples were isolated on violet-red bile glucose agar. A total of 197 isolates were screened for the presence of the irp2 gene, encoded within the HPI, by PCR. One isolate that was positive for irp2 gene was also positive for the fyuA, irp1, ybtP/ybtQ, ybtX/ybtS and int/asn tRNA genes by PCR. The presence of fyuA, irp1 and irp2 genes was confirmed by Southern hybridization.

CONCLUSIONS

The isolate was identified as Serratia liquefaciens by sequencing of the 16S rRNA gene and by ribotyping.

SIGNIFICANCE AND IMPACT OF THE STUDY

This is the first report of a Serratia harbouring the Yersinia HPI. Serratia is a frequently occurring Enterobacteriaceae genus in chill-stored meat.

摘要

目的

本研究旨在筛查肉类中的肠杆菌科菌群，以检测携带耶尔森氏菌高致病性岛（HPI）的细菌。

方法与结果

从29份肉类和29份肝脏样本中分离出的细菌在紫红胆盐葡萄糖琼脂上培养。通过PCR对总共197株分离菌进行HPI编码的irp2基因检测。一株irp2基因呈阳性的分离菌，其fyuA、irp1、ybtP/ybtQ、ybtX/ybtS和int/asn tRNA基因通过PCR检测也呈阳性。通过Southern杂交证实了fyuA、irp1和irp2基因的存在。